Histone deacetylase HDA-2 regulates Trichoderma atroviride growth, conidiation, blue light perception, and oxidative stress responses

dc.contributorAmerican Society for Microbiology
dc.creatorOsorio Concepción, Macario
dc.creatorCristóbal Mondragón, Gema Rosa
dc.creatorGutiérrez Medina, Braulio
dc.creatorCasas Flores, J. Sergio
dc.date2018-07-11T18:30:05Z
dc.date2018-07-11T18:30:05Z
dc.date2017-02
dc.date.accessioned2023-07-17T22:03:29Z
dc.date.available2023-07-17T22:03:29Z
dc.identifierOsorio-Concepción, M., Cristóbal-Mondragón, G. R., Gutiérrez-Medina, B., & Casas-Flores, S. (2017). Histone Deacetylase HDA-2 Regulates Trichoderma atroviride Growth, Conidiation, Blue Light Perception, and Oxidative Stress Responses. Applied and Environmental Microbiology, 83(3), e02922–16. http://doi.org/10.1128/AEM.02922-16
dc.identifierhttp://hdl.handle.net/11627/4040
dc.identifierhttp://doi.org/10.1128/AEM.02922-16
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7543527
dc.description"Fungal blue-light photoreceptors have been proposed as integrators of light and oxidative stress. However, additional elements participating in the integrative pathway remain to be identified. In Trichoderma atroviride, the blue-light regulator (BLR) proteins BLR-1 and -2 are known to regulate gene transcription, mycelial growth, and asexual development upon illumination, and recent global transcriptional analysis revealed that the histone deacetylase-encoding gene hda-2 is induced by light. Here, by assessing responses to stimuli in wild-type and ?hda-2 backgrounds, we evaluate the role of HDA-2 in the regulation of genes responsive to light and oxidative stress. ?hda-2 strains present reduced growth, misregulation of the con-1 gene, and absence of conidia in response to light and mechanical injury. We found that the expression of hda-2 is BLR-1 dependent and HDA-2 in turn is essential for the transcription of early and late light-responsive genes that include blr-1, indicating a regulatory feedback loop. When subjected to reactive oxygen species (ROS), ?hda-2 mutants display high sensitivity whereas ?blr strains exhibit the opposite phenotype. Consistently, in the presence of ROS, ROS-related genes show high transcription levels in wild-type and ?blr strains but misregulation in ?hda-2 mutants. Finally, chromatin immunoprecipitations of histone H3 acetylated at Lys9/Lys14 on cat-3 and gst-1 promoters display low accumulation of H3K9K14ac in ?blr and ?hda-2 strains, suggesting indirect regulation of ROS-related genes by HDA-2. Our results point to a mutual dependence between HDA-2 and BLR proteins and reveal the role of these proteins in an intricate gene regulation landscape in response to blue light and ROS."
dc.formatapplication/pdf
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rightsAcceso Abierto
dc.subjectTrichoderma
dc.subjectBlue light
dc.subjectOxidative stress
dc.subjectBLR proteins
dc.subjectHistone acetylation
dc.subjectHistone deacetylases
dc.subjectHDA-2
dc.subjectHos2p
dc.subjectCIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA
dc.titleHistone deacetylase HDA-2 regulates Trichoderma atroviride growth, conidiation, blue light perception, and oxidative stress responses
dc.typearticle


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