| dc.contributor | Hernández Castillo, Francisco Daniel | |
| dc.contributor | Jasso Cantú, Diana | |
| dc.contributor | Arredondo Valdés, Roberto | |
| dc.contributor | Castillo Reyes, Francisco | |
| dc.creator | Fernández Guzmán, Patricia | |
| dc.date | 2017-12-01 | |
| dc.date.accessioned | 2023-07-17T20:58:15Z | |
| dc.date.available | 2023-07-17T20:58:15Z | |
| dc.identifier | http://repositorio.uaaan.mx:8080/xmlui/handle/123456789/42909 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/7526885 | |
| dc.description | "Picea mexicana Martínez especie endémica de México en peligro de extinción amenazada por diversos factores, entre estos; plagas y enfermedades como es el caso de la pudrición de raíz por Fusarium oxysporum ocasionando perdida de 60 % o más en plántula. El uso de extractos vegetales es una alternativa frente al uso de fungicidas químicos que son potencialmente dañinos y generan cepas resistentes. En el presente trabajo se evaluó in vitro e in vivo la actividad antifúngica de extractos vegetales de Larrea tridentata, Lippia graveolens, Agave lechuguilla, Jatropha dioica y Carya illinoinensis contra Fusarium oxysporum agente causal de la pudrición de raíz en Picea. El ensayo in vitro se realizó mediante micro dilución en placas. El bioensayo in vivo se realizó
xi
aplicando los extractos que obtuvieron mayor actividad antifúngica in vitro a semillas de Picea en placas Petri con medio MS. Se evaluó la incidencia del patógeno así como el porcentaje de germinación. El extracto etanólico de Lippia graveolens tallo fue el que obtuvo mejores resultados in vitro alcanzando hasta un 97 % de inhibición en el crecimiento de Fusarium oxysporum, sin embargo se encontró que los extractos acuosos no presentaron actividad antifúngica. En el bioensayo in vivo el mejor tratamiento fue el de Agave lechuguilla hoja etanólico, reduciendo la incidencia del patógeno y favoreciendo en un 65 % la germinación de la semilla." | |
| dc.description | ABSTRACT:
Xylella fastidiosa is a pathogenic bacterium of plants, which in recent years has acquired considerable importance due to the immense range of susceptible hosts that affects, currently there are no efficient control measures. Many countries have been affected by the diseases caused, for example: In theUnited States by the disease of Pierce in the vine, Brazil, by the variegated chlorosis of citrus, Italy by the syndrome of sudden decay of the olive tree. In Mexico, Pierce's disease has been detected in three grape-producing regions. The bacterium has also been detected in other hosts such as: coffee, apricot, peach, ash, flower laurel, among others. To create specific and efficient control measures, it is necessary to know the vectors and hosts of the bacteria. The project was carried out in the area surrounding the vineyards of Parras de la Fuente, Coahuila, where crop leaves, ornamental plants, fruit and forest trees, grasses and adjacent weeds were collected, with and without symptoms similar to the disease of Pierce, and at the same time insects were collected through the use of an entomological network and manual collection, in order to find alternate hosts and Xylella
viii
fastidiosa vectors. In total, 22 species of plants of different taxonomic families were collected. The diagnosis of X. fastidiosa in plants was made by PCR tests using specific primers for fastidiosa subspecie, using only the central venation of the leaves and part of the petiole. Of the insects collected, six genera and four species of leafhoppers were identified through taxonomic keys and extraction of genitalia; the diagnosis of X. fastidiosa in insects, was made by PCR tests with specific primers for annoying subspecies, in these tests a maceration of the whole body of the insect was used. X. fastidiosa was detected in Homalodisca vitripennis, in commercial and wild vine plants, apricot and ash trees; the subspecies found in vine corresponds to X. fastidiosa subsp. fastidiosa, while in apricot and ash an X. fastidiosa subsp. multiplex. | |
| dc.format | PDF | |
| dc.language | Español | |
| dc.rights | Acceso Abierto | |
| dc.rights | CC BY-NC-ND - Atribución-NoComercial-SinDerivadas | |
| dc.subject | Peligro de extinción | |
| dc.subject | Inhibición | |
| dc.subject | Dilución en Micro-Placa. | |
| dc.subject | CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA | |
| dc.title | Actividad antifúngica de extractos vegetales para el control de fusarium oxysporum en la pudrición de raíz de picea mexicana | |
| dc.type | Tesis de maestría | |
| dc.type | Versión publicada | |
| dc.audience | Estudiantes | |
| dc.audience | Investigadores | |
