Construcción de plásmidos del virus de Chikungunya, de replicación y ayuda para la transfección de células de mamíferos

Construction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells

dc.contributorComas García, Mauricio
dc.contributorCastillo Martín del Campo, Claudia G.
dc.contributorRosales Mendoza, Sergio
dc.contributorBernal Silva, Sofía
dc.contributorArgüello Astorga, Gerardo Rafael
dc.contributorMAURICIO COMAS GARCIA;221633
dc.contributorCLAUDIA GUADALUPE CASTILLO MARTIN DEL CAMPO;217265
dc.contributorSERGIO ROSALES MENDOZA;102163
dc.contributorSOFIA BERNAL SILVA;165702
dc.contributorGerardo Rafael Argüello Astorga;12605
dc.creatorMayra Colunga-Saucedo;0000-0001-9219-2617
dc.creatorColunga Saucedo, Mayra
dc.date2023-05-02T14:50:39Z
dc.date2023-05-02T14:50:39Z
dc.date2023-05-02
dc.date.accessioned2023-07-17T20:32:13Z
dc.date.available2023-07-17T20:32:13Z
dc.identifierhttps://repositorioinstitucional.uaslp.mx/xmlui/handle/i/8261
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/7517291
dc.descriptionThe genome of Alphaviruses can be modified to produce self-replicating RNAs and virus-like particles, which are useful virological tools. In this work, we generated three plasmids for the transfection of mammalian cells: an infectious clone of Chikungunya virus (CHIKV), one that codes for the structural proteins (helper plasmid), and another one that codes nonstructural proteins (replicon plasmid). All of these plasmids contain a reporter gene (mKate2). The reporter gene in the replicon RNA and the infectious clone are synthesized from subgenomic RNA. Co-transfection with the helper and replicon plasmids has biotechnological/biomedical applications because they allow for the delivery of self-replicating RNA for the transient expression of one or more genes to the target cells.
dc.descriptionUASLP-PTC-622 (M.C.G.), UASLP-FAI-2018-14 (M.C.G.) y CONACYT-321364 (S.R.M.)
dc.descriptionInvestigadores
dc.descriptionEstudiantes
dc.formatapplication/pdf
dc.languageInglés
dc.publisherFacultad de Medicina
dc.relationREPOSITORIO NACIONAL CONACYT
dc.relationViruses 2023, 15, 132. https://doi.org/10.3390/v15010132
dc.rightsAcceso Abierto
dc.rightshttp://creativecommons.org/licenses/by/4.0
dc.subjectChikungunya virus
dc.subjectAlphavirus DNA vectors
dc.subjectReplicon plasmid
dc.subjectHelper plasmid
dc.subjectVirus Chikungunya (bvs)
dc.subjectAlphavirus (bvs)
dc.subjectPlásmidos (bvs)
dc.subjectMEDICINA Y CIENCIAS DE LA SALUD
dc.titleConstrucción de plásmidos del virus de Chikungunya, de replicación y ayuda para la transfección de células de mamíferos
dc.titleConstruction of a Chikungunya Virus, Replicon, and Helper Plasmids for Transfection of Mammalian Cells
dc.typeTesis de doctorado
dc.coverageMéxico. San Luis Potosí. San Luis Potosí


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