Micronutrient deficiency is one of the major widespread problem in the population caused by hidden hunger, in order to address these challenges various biofortification programmes have been designed worldwide. In this study kernel concentrations of iron and zinc were evaluated in two MPS population consisting of 193 double haploid lines using Atomic Absorption Spectrophotometer (AAS) method. Bioavailability of iron and zinc in these double haploid lines were found using the phytate concentration obtained through wade assay method. The Phy/Fe and Phy/Zn molar ratios in MPS 1 population ranged from 0.33 to 63.93 and from 0.53 to 80.99, respectively. In MPS 2 population Phy/Fe and Phy/Zn molar ratios ranged from 0.28 to 73.34 and from 0.30 to 83.80, respectively. According to the phy/Fe and phy/Zn molar ratios, the lines ZL19406, ZL19365 and Zl19412 were found to have potential for the development of cultivars of maize containing high zinc and iron concentration.160-165