Flour yellowness b* is a major criterion to estimate flour colour, which is basically determined by yellow pigment content in wheat grains. A doubled haploid (DH) population with 71 lines derived from a cross between Zhongyou 9507 with lower flour yellowness b* and CA9632 with higher flour yellowness b*, was sown for two years in 5 locations. A total of 173 loci including 122 SSR, 4 STS of storage protein genes and 10 AFLP markers were used to construct a linkage map, covering 2 881 cM of whole genome at 21 linkage groups. With the method of composite interval mapping (CIM), 8 putative major QTL of yellowness b* were detected on chromosomes 1DS, 2DL, 3A, 4D, 5D, 6AL, 6D and 7AL (Table 2), and 5 putative QTL of yellow pigment content were detected on chromosome 2DL, 3DL, 4A, 5A and 7AL (Table 3), respectively. The QTL on 7AL was common for yellowness b* and yellow pigment content, accounting for 12.9%–37.6% and 12.1%–33.9% of the phenotypic variance across different environments, respectively, which was closely linked to Xgwm264b. The genetic distance between Xgwm264b and the QTL of flour yellowness b* and grain yellow pigment on chromosome 7AL were 0–3.9 cM and 0–0.9 cM, respectively. It can be used as a molecular marker in wheat breeding program.41-45