The Mediterranaen cereal cyst nematode, Heterodera latipons, causing serious yield losses in protected cultivation cereal cropping areas in Turkey. An accurate and reliable identification is primarily needed to establish effective, sustainable and environmentally safe control measures to any cyst nematode species. In this study, twenty-five populations of Heterodera latipons collected from cereal areas in East-Mediterranean and South-East Anatolian regions were identified using species-specific primers (SCAR). The SCAR primer pair, H-latac F and H-latact R, defined species-specific primers were used to identify Heterodera populations. In this study stated that H-latac F and H-latact R primers for identifying of H. latipons can be efficient tools to identify the Turkish Mediterranean cereal cyst nematode populations. Moreover, the results indicated that H. latipons was the prominent cereal cyst nematode species in the East Mediterranean and South East Anatolian regions of Turkey. This clarified approach may supply a quick basis for identification of H. latipons populations for their future management and facilitate checking its distribution in the agricultural areas.90-95