| dc.contributor | de Coss, A.L., Cuerpo Académico Ganadería Tropical Sustentable, Facultad de Ciencias Agrícolas, Universidad Autónoma de Chiapas, Entronque Carretera Costera S/N, Huehuetán, Chiapas. CP. 36670, Mexico; Tinajero, J.J.M., Cuerpo Académico Ganadería Tropical Sustentable, Facultad de Ciencias Agrícolas, Universidad Autónoma de Chiapas, Entronque Carretera Costera S/N, Huehuetán, Chiapas. CP. 36670, Mexico; Agreda, F.J.M., Cuerpo Académico Ganadería Tropical Sustentable, Facultad de Ciencias Agrícolas, Universidad Autónoma de Chiapas, Entronque Carretera Costera S/N, Huehuetán, Chiapas. CP. 36670, Mexico; Castillo, C.G.G., Cuerpo Académico Ganadería Tropical Sustentable, Facultad de Ciencias Agrícolas, Universidad Autónoma de Chiapas, Entronque Carretera Costera S/N, Huehuetán, Chiapas. CP. 36670, Mexico; Valdez, O.D.M., Departamento de Desarrollo Regional, Centro Universitario del Sur, Universidad de Guadalajara, Zapotlán el Grande, Jalisco, Mexico; Medina, E.G., Departamento de Producción Agrícola, Universidad de Guadalajara, Autlán, Jalisco, Mexico | |
| dc.description.abstract | The present study was carried out to evaluate defaunant effect of the water soluble extracts of the plants Buddleia cordata, Chenopodium ambrosioides, Datura inoxia, Marrubium vulgare, Mentha piperita and Verbesina perymenioides, which contain secondary compounds with possible toxic effect on rumen ciliate protozoa. The defaunant property was estimated inoculating 0.5 mL of a concentrate of protozoa in 9.5 mL of an anaerobic culture medium (ACM) enriched with the soluble extract of Avena sativa (control treatment), which was added 150 μL of water-soluble extract of the evaluated plants, in a design completely at random. The quantification of ciliate protozoa was done by means of the direct count to 40X in a Neubaüer chamber, at 0, 24, 48 and 72 h. Defaunant capacity (DC) was classified as high (DCA), medium (DCM) and null (DCN). The ACM supported even the 72 h of incubation a concentration of protozoa of 104 mL-1 culture media, with 80% of viability. The extracts of C. ambrosioides, M. vulgare and M. piperita reduced (P<0.05) the quantity of protozoa to less than 102 cell mL-1 of culture medium between 24 and 72 h of incubation, it was DCA; whereas, the extract of the plants V. perymenioides and B. cordata, they showed a DCM and only up DCA to 72 h of incubation (P<0.05). D. Inoxia showed DCN at 72 h of incubation was similar to control. The concentration of total and cellulolytic bacteria was unaffected by the extract that had CDA plants, more research is needed to identify the type and concentration of desfaunant substances in C. ambrosioides, M. vulgare and M. piperita, besides the degree of toxicity that can cause these substances to ruminants. | |
