Percentage of ubiquitinated spermatozoa does not correlate with fertilizing capacity of thawed bovine semen

Abstract

Laboratory tests in inflammatory myopathies (IIM) are of great help in the diagnosis of these diseases. Two main groups can be defined, one of them quantifies the muscle enzymes that reflect muscle inflammation and the other one detects the presence of autoantibodies which reflect the autoimmune process in these diseases. The most important muscle enzyme is creatine kinase and other enzymes to take into consideration are aspartate and alanine aminotransferase, aldolase and lactic dehydrogenase. In the autoantibodies group, antinuclear antibodies are the most important, since they occur in approximately 50-80% of patients with IIM and help define the distinct disease sub-groups. They are divided into myositis specific antibodies (MSA) and myositis associated antibodies (MAA). The most important MSA are anti-Jo-1 antibody which occur in patients with Polymyositis and anti-Mi-2 antibody that occur in patients with Dermatomyositis. " 2009 Elsevier España, S.L. All rights reserved.",,,,,,"10.1016/j.reuma.2009.09.005",,,"http://hdl.handle.net/20.500.12104/42469","http://www.scopus.com/inward/record.url?eid=2-s2.0-72149097310&partnerID=40&md5=e0bc09bf30a38208c5f59510181d71fa",,,,,,"SUPPL. 3",,"Reumatologia Clinica",,"16

19",,"5",,"Scopus",,,,,,"Antinuclear antibodies; Autoantibodies in myositis; Enzymes in myositis; Myositis related laboratory abnormalities; Myositis specific antibodies",,,,,,"Laboratory abnormalities and autoantibodies [Alteraciones de laboratorio y autoanticuerpos]",,"Article" "45299","123456789/35008",,"Rodríguez-Lozano, I., Laboratorio de Bioquímica de la Reproducción, Departamento de Producción agrícola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, MEX City, Mexico; Ávalos-Rodríguez, A., Laboratorio de Bioquímica de la Reproducción, Departamento de Producción agrícola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, MEX City, Mexico; Castillo-Juárez, H., Laboratorio de Bioquímica de la Reproducción, Departamento de Producción agrícola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, MEX City, Mexico; Borderas-Tordesillas, F., Laboratorio de Bioquímica de la Reproducción, Departamento de Producción agrícola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, MEX City, Mexico; Roa-Vidal, J.J., Laboratorio de Embriología Experimental, Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Guadalajara, Mexico; Rosales-Torres, A.M., Laboratorio de Bioquímica de la Reproducción, Departamento de Producción agrícola y Animal, Universidad Autónoma Metropolitana, Unidad Xochimilco, MEX City, Mexico",,"Rodriguez-Lozano, I.

Avalos-Rodriguez, A.

Castillo-Juarez, H.

Borderas-Tordesillas, F.

Roa-Vidal, J.J.

Rosales-Torres, A.M.",,"2014",,"Contents: In the spermatozoa of some species, the ubiquitin-proteasome system detects altered proteins and tags them for elimination by the proteasome. In some species' ejaculates, a high proportion of ubiquitinated spermatozoa (i.e. those having ubiquitin bound to the altered or damaged membrane proteins) has been related to infertility. The aim of this study was to assess whether the percentage of ubiquitinated spermatozoa relates to fertility of dairy bulls and whether ubiquitination increases during protein remodelling that occurs during in vitro spermatic capacitation. Thirty-two frozen semen straws from four high-fertility (ReproMax ) and four normal-fertility (Normal) Holstein-Friesian sires were evaluated. Ubiquitinated and capacitated spermatozoa were quantified by sperm ubiquitin tag immunoassay and chlortetracycline stain, respectively. Fertilizing capacity of sires was assessed by in vitro fertilization. No differences were found between Normal and ReproMax sires with regard to the observed percentage of ubiquitinated spermatozoa (42.97 3.69% and 49.68 9.27%, respectively; p > 0.05). Additionally, no differences were found in the percentage of ubiquitinated spermatozoa as a consequence of spermatic capacitation in either Normal (42.97 3.69% before capacitation vs 44.67 7.5% after; p > 0.05) or ReproMax sires (49.68 9.27% before vs 45.05 7.51% after; p > 0.05). The percentage of ubiquitinated spermatozoa in a thawed sperm samples did not correlate with its in vitro fertilizing capacity; thus, this assay does not prove useful to detect in vivo fertility differences between sires. Additionally, protein degradation occurring during remodelling of the spermatozoon plasma membrane during the capacitation process does not seem to involve the ubiquitin-proteasome system. " 2013 Blackwell Verlag GmbH.