Papel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos

dc.contributorMendivil Anaya, Carlos Olimpo
dc.contributorDiabetes, Lípidos y Metabolismo UniAndes
dc.contributorPaula Valentina Gaete Carrillo, 0000-0001-9387-6017
dc.contributorhttps://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000097625
dc.contributorhttps://www.researchgate.net/profile/Paula-Gaete
dc.contributorhttps://scholar.google.com/citations?user=Wd5n4WQAAAAJ&hl=es&authuser=1
dc.creatorGaete Carrillo, Paula Valentina
dc.date.accessioned2023-02-22T15:39:50Z
dc.date.accessioned2023-06-06T23:39:02Z
dc.date.available2023-02-22T15:39:50Z
dc.date.available2023-06-06T23:39:02Z
dc.date.created2023-02-22T15:39:50Z
dc.date.issued2023-02-21
dc.identifierhttps://repositorio.unal.edu.co/handle/unal/83542
dc.identifierUniversidad Nacional de Colombia
dc.identifierRepositorio Institucional Universidad Nacional de Colombia
dc.identifierhttps://repositorio.unal.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6651426
dc.description.abstractPapel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos Objetivo: El objetivo de esta investigación es esclarecer la participación del receptor nuclear PPARβ/δ en la respuesta metabólica que desarrollan los macrófagos de ratón después de la exposición a ácidos grasos de cadena media (octanoico, C8:0 y decanoico, C10:0). Metodología: Se cultivaron macrófagos murinos J774A.1 en diferentes condiciones de tratamiento; en la mitad de las células se realizó un knock-down de la expresión para PPARβ/δ con un sistema de ácido ribonucleico pequeño de interferencia y el porcentaje de interferencia obtenido se verificó por RT-qPCR. El diseño experimental comparó 5 factores en las células a estudio: - Knockdown de PPARβ/δ (sí o no) - Exposición a ácido graso (cadena media vs cadena larga) - Exposición a un agonista de PPARβ/δ (GW501516) - Activación por lipopolisacárido (sí o no) - Control negativo En cada grupo de células se evaluó la expresión de genes relacionados con el metabolismo lipídico (Scarb1, Plin2, Npc1, Pltp, Cpt1, Abca1 y Abcg1) y con la función inmunitaria (Il6, Tnf, Ifng, Il10, Nos2, Ptgs2, Mcp1y Mmp9) mediante RT-qPCR. La expresión de cada gen se cuantificó de forma relativa al gen de la gliceraldeheido-3-fosfato deshidrogenasa utilizando el método ∆∆Ct. Para evaluar el impacto funcional de la exposición a ácidos grasos de cadena media sobre la fagocitosis de lipoproteínas, un paso fundamental de la aterogénesis, se realizó en cada subgrupo de tratamientos un ensayo de captación de lipoproteínas de baja densidad (LDL) fluorescentemente marcadas. Resultados y conclusiones: Este estudio amplió el conocimiento sobre la respuesta a los ácidos grasos de cadena media en la expresión de genes relacionados con el metabolismo de lípidos y la función inmune en macrófagos murinos, y en qué medida este efecto está mediado por el receptor nuclear PPARβ/δ. Palabras clave: Ppard, metabolismo, lípidos, colesterol, ateroesclerosis e inflamación. (Texto tomado de la fuente)
dc.description.abstractRole of the nuclear receptor PPARβ/δ in the metabolic response to medium-chain fatty acids in murine macrophages Aim: The aim of this investigation is to clarify the role of the nuclear receptor PPARβ/δ in the metabolic response that the murine macrophages develop after the exposition to medium-chain fatty acids (octanoic acid, C8:0 y decanoic acid, C10:0). Methods: J774A.1 murine macrophages were cultured under different treatment conditions; Half of the cells, had a knock-down against PPARβ/δ by using small interfering ribonucleic acids and the percentage of reduced expression was verified by RT-qPCR. The experimental design compared 5 factors in the cells under study: - PPARβ/δ knockdown (yes or no) - Exposure to fatty acid (medium chain vs. long chain) - Exposure to a PPARβ/δ agonist (GW501516) - Activation by lipopolysaccharide (yes or no) - Negative control The expression of genes related to lipid metabolism (Scarb1, Plin2, Npc1, Pltp, Cpt1, Abca1 and Abcg1) and immune function (Il6, Tnf, Ifng, Il10, Nos2, Ptgs2, Mcp1 and Mmp9) was evaluated in each group of cells by RT-qPCR. The expression of each gene was quantified relative to the glyceraldehyde-3-phosphate dehydrogenase gene using the ∆∆Ct method. To assess the functional impact of medium-chain fatty acid exposure on lipoprotein phagocytosis, a critical step in atherogenesis, a fluorescently labeled low-density lipoprotein (LDL) uptake assay was performed on each treatment subgroup. Conclusions and results: This study furthered our understanding of the response to medium-chain fatty acids in the expression of genes related to lipid metabolism and immune function in murine macrophages, and clarify the role of the nuclear receptor PPARβ/δ in this process. Key words: Ppard, metabolism, lipids, cholesterol, atherosclerosis and inflammation.
dc.languagespa
dc.publisherUniversidad Nacional de Colombia
dc.publisherBogotá - Medicina - Maestría en Bioquímica
dc.publisherFacultad de Medicina
dc.publisherBogotá, Colombia
dc.publisherUniversidad Nacional de Colombia - Sede Bogotá
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dc.rightsAtribución-NoComercial 4.0 Internacional
dc.rightshttp://creativecommons.org/licenses/by-nc/4.0/
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titlePapel del receptor nuclear PPARβ/δ en la respuesta metabólica a ácidos grasos de cadena media en macrófagos murinos
dc.typeTrabajo de grado - Maestría


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