dc.creatorBaez-Jurado, Eliana
dc.creatorGuio-Vega, Gina
dc.creatorHidalgo-Lanussa, Oscar
dc.creatorGonzález, Janneth
dc.creatorEcheverria, Valentina
dc.creatorAshraf, Ghulam Md
dc.creatorSahebkar, Amirhossein
dc.creatorBarreto, George E.
dc.date.accessioned2020-11-03T07:35:07Z
dc.date.accessioned2023-05-30T20:44:55Z
dc.date.available2020-11-03T07:35:07Z
dc.date.available2023-05-30T20:44:55Z
dc.date.created2020-11-03T07:35:07Z
dc.date.issued2019
dc.identifier0893-7648
dc.identifierhttp://repositorio.uss.cl/xmlui/handle/uss/165
dc.identifierhttp://dx.doi.org/10.1007/s12035-018-1442-9
dc.identifier1559-1182
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/6446124
dc.description.abstractAstrocytes are specialized cells capable of regulating inflammatory responses in neurodegenerative diseases or traumatic brain injury. In addition to playing an important role in neuroinflammation, these cells regulate essential functions for the preservation of brain tissue. Therefore, the search for therapeutic alternatives to preserve these cells and maintain their functions contributes in some way to counteract the progress of the injury and maintain neuronal survival in various brain pathologies. Among these strategies, the conditioned medium from human adipose-derived mesenchymal stem cells (CM-hMSCA) has been reported with a potential beneficial effect against several neuropathologies. In this study, we evaluated the potential effect of CM-hMSCA in a model of human astrocytes (T98G cells) subjected to scratch injury. Our findings demonstrated that CM-hMSCA regulates the cytokines IL2, IL-6, IL-8, IL-10, GM-CSF, and TNF-a, downregulates calcium at the cytoplasmic level, and regulates mitochondrial dynamics and the respiratory chain. These actions are accompanied by modulation of the expression of different proteins involved in signaling pathways such as AKT/pAKT and ERK1/2/pERK, and may mediate the localization of neuroglobin (Ngb) at the cellular level. We also confirmed that Ngb mediated the protective effects of CM-hMSCA through regulation of proteins involved in survival pathways and oxidative stress. In conclusion, regulation of brain inflammation combined with the recovery of fundamental cellular aspects in the face of injury makes CM-hMSCA a promising candidate for the protection of astrocytes in brain pathologies.
dc.languageen
dc.publisherFacultad de Ciencias de la Salud
dc.relationvol. 56, no. 7, p. 5167-5187
dc.relationIndexado en WOS
dc.rightshttps://creativecommons.org/licenses/by-nc-nd/3.0/cl/
dc.rightsAtribución-NoComercial-SinDerivadas 3.0 Chile
dc.sourceMolecular Neurobiology
dc.subjectASTROCYTES
dc.subjectSCRATCH ASSAY
dc.subjectMESENCHYMAL STEM CELLS
dc.subjectINFLAMMATION
dc.subjectCONDITIONED MEDIUM
dc.subjectNEUROGLOBIN
dc.subjectHUMAN BONE-MARROW
dc.subjectOXYGEN-GLUCOSE DEPRIVATION
dc.subjectCENTRAL-NERVOUS-SYSTEM
dc.subjectMETHYL-D-ASPARTATE
dc.subjectIN-VITRO
dc.subjectOXIDATIVE STRESS
dc.subjectGROWTH-FACTOR
dc.subjectSIGNALING PATHWAY
dc.subjectSTROMAL CELLS
dc.subjectGLUTAMATE EXCITOTOXICITY
dc.titleMitochondrial Neuroglobin Is Necessary for Protection Induced by Conditioned Medium from Human Adipose-Derived Mesenchymal Stem Cells in Astrocytic Cells Subjected to Scratch and Metabolic Injury
dc.typeArticle


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