dc.creator | Universidad San Sebastián | |
dc.creator | Universidad San Sebastián | |
dc.creator | Universidad San Sebastián | |
dc.creator | Universidad San Sebastián | |
dc.creator | Baez-Jurado, Eliana | |
dc.creator | Vega, Gina Guio | |
dc.creator | Aliev, Gjumrakch | |
dc.creator | Tarasov, Vadim V. | |
dc.creator | Esquinas, Paula | |
dc.creator | Echeverria, Valentina | |
dc.creator | Barreto, George E. | |
dc.date.accessioned | 2023-05-24T04:21:34Z | |
dc.date.available | 2023-05-24T04:21:34Z | |
dc.date.created | 2023-05-24T04:21:34Z | |
dc.date.issued | 2018-03-01 | |
dc.identifier | 0893-7648 | |
dc.identifier | https://repositorio.uss.cl/handle/uss/3808 | |
dc.identifier | 10.1007/s12035-017-0481-y | |
dc.description.abstract | Previous studies have indicated that paracrine factors (conditioned medium) increase wound closure and reduce reactive oxygen species in a traumatic brain injury in vitro model. Although the beneficial effects of conditioned medium from human adipose tissue-derived mesenchymal stem cells (hMSCA-CM) have been previously suggested for various neurological diseases, their actions on astrocytic cells are not well understood. In this study, we have explored the effect of hMSCA-CM on human astrocyte model (T98G cells) subjected to scratch assay. Our results indicated that hMSCA-CM improved cell viability, reduced nuclear fragmentation, attenuated the production of reactive oxygen species, and preserved mitochondrial membrane potential and ultrastructural parameters. In addition, hMSCA-CM upregulated neuroglobin in T98G cells and the genetic silencing of this protein prevented the protective action of hMSCA-CM on damaged cells, suggesting that neuroglobin is mediating, at least in part, the protective effect of hMSCA-CM. Overall, this evidence suggests that the use of hMSCA-CM is a promising therapeutic strategy for the protection of astrocytic cells in central nervous system (CNS) pathologies. | |
dc.language | eng | |
dc.relation | Molecular Neurobiology | |
dc.title | Blockade of Neuroglobin Reduces Protection of Conditioned Medium from Human Mesenchymal Stem Cells in Human Astrocyte Model (T98G) Under a Scratch Assay | |
dc.type | Artículo | |