dc.creator | Ugarte, Francisca | |
dc.creator | Santapau, Daniela | |
dc.creator | Gallardo, Vivian | |
dc.creator | Garfias, Carolina | |
dc.creator | Yizmeyián, Anahí | |
dc.creator | Villanueva, Soledad | |
dc.creator | Sepúlveda, Carolina | |
dc.creator | Rocco, Jocelyn | |
dc.creator | Pasten, Consuelo | |
dc.creator | Urquidi, Cinthya | |
dc.creator | Cavada, Gabriel | |
dc.creator | San Martin, Pamela | |
dc.creator | Cano, Francisco | |
dc.creator | Irarrázabal, Carlos E. | |
dc.date.accessioned | 2022-03-28T15:00:26Z | |
dc.date.accessioned | 2023-05-19T14:47:16Z | |
dc.date.available | 2022-03-28T15:00:26Z | |
dc.date.available | 2023-05-19T14:47:16Z | |
dc.date.created | 2022-03-28T15:00:26Z | |
dc.date.issued | 2021 | |
dc.identifier | Ugarte F, Santapau D, Gallardo V, Garfias C, Yizmeyia´ n A, Villanueva S, Sepu´lveda C, Rocco J, Pasten C, Urquidi C, Cavada G, San Martin P, Cano F and Irarra´ zabal CE (2022) Urinary Extracellular Vesicles as a Source of NGAL for Diabetic Kidney Disease Evaluation in Children and Adolescents With Type 1 Diabetes Mellitus. Front. Endocrinol. 12:654269. doi: 10.3389/fendo.2021.654269 | |
dc.identifier | https://doi.org/10.3389/fendo.2021.654269 | |
dc.identifier | http://hdl.handle.net/11447/5821 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/6301876 | |
dc.description.abstract | Background: Tubular damage has a role in Diabetic Kidney Disease (DKD). We evaluated
the early tubulointerstitial damage biomarkers in type-1 Diabetes Mellitus (T1DM) pediatric
participants and studied the correlation with classical DKD parameters.
Methods: Thirty-four T1DM and fifteen healthy participants were enrolled. Clinical and
biochemical parameters [Glomerular filtration Rate (GFR), microalbuminuria (MAU),
albumin/creatinine ratio (ACR), and glycated hemoglobin A1c (HbA1c)] were evaluated.
Neutrophil gelatinase-associated lipocalin (NGAL), Hypoxia-inducible Factor-1a (HIF-1a),
and Nuclear Factor of Activated T-cells-5 (NFAT5) levels were studied in the supernatant
(S) and the exosome-like extracellular vesicles (E) fraction from urine samples.
Results: In the T1DM, 12% had MAU >20 mg/L, 6% ACR >30 mg/g, and 88% had eGFR
>140 ml/min/1.72 m2
. NGAL in the S (NGAL-S) or E (NGAL-E) fraction was not detectable
in the control. The NGAL-E was more frequent (p = 0.040) and higher (p = 0.002) than
NGAL-S in T1DM. The T1DM participants with positive NGAL had higher age (p = 0.03),
T1DM evolution (p = 0.03), and serum creatinine (p = 0.003) than negative NGAL. The
NGAL-E correlated positively with tanner stage (p = 0.0036), the median levels of HbA1c
before enrollment (p = 0.045) and was independent of ACR, MAU, and HbA1c at the
enrollment. NFAT5 and HIF-1a levels were not detectable in T1DM or control.
Conclusion: Urinary exosome-like extracellular vesicles could be a new source of early
detection of tubular injury biomarkers of DKD in T1DM patients. | |
dc.language | en | |
dc.subject | Diabetic kidney disease | |
dc.subject | Type 1 diabetes mellitus | |
dc.subject | Children | |
dc.subject | NGAL | |
dc.subject | Urinary extracellular vesicles | |
dc.title | Urinary Extracellular Vesicles as a Source of NGAL for Diabetic Kidney Disease Evaluation in Children and Adolescents With Type 1 Diabetes Mellitus | |
dc.type | Article | |