Dissertação
Processo de conversão de substrato renovável por aspergillus terreus UCP 0076 na biossíntese do inibidor da HMG-CoA redutase e intermediários.
Fecha
2021-03-08Registro en:
LINS, Uiara Maria de Barros Lira. Processo de conversão de substrato renovável por aspergillus terreus UCP 0076 na biossíntese do inibidor da HMG-CoA redutase e intermediários. 2021. 112 f. : il. Dissertação (Mestrado) - Universidade Católica de Pernambuco. Programa de Pós-graduação em Desenvolvimento de Processos Ambientais. Mestrado em Desenvolvimento de Processos Ambientais, 2021.
Autor
Lins, Uiara Maria de Barros Lira
Resumen
HMG-CoA reductase (3-hydroxy-3-methylglutaryl-coenzyme reductase) is the enzyme
that has a limiting role in cholesterol biosynthesis. Among the HMG-CoA reductase
inhibitory molecules, statins stand out, such as simvastatin, a semi-synthetic drug used
to reduce high cholesterol levels, being one of the most sold statins in the world.
Currently, the industrial production of simvastatin occurs in a process consisting of
several stages, starting from the natural product lovastatin. Studies were carried out
with the objective of evaluating the biotechnological potential of Aspergillus terreus
UCP0076 in the production of HMG-CoA reductase inhibitors and intermediates by
submerged fermentation (FS), using agro-industrial substrate, through innovative
processes. The first invention corresponded to the submerged fermentation process,
using the culture media RCMS (solid cassava husk residue) and RCMC (cassava husk
residue broth) consisting of cassava husks as a renewable raw material. The second
refers to the development of a solid (RCMS) and liquid (RCMC) culture medium, the
base of the waste disposal of cassava husks (Manihot esculenta), which provides high
availability of nutrients that favor the growth of filamentous fungi and secondary
metabolite production. At the end of fermentation, the product obtained corresponded
to a biomolecule with a structure similar to simvastatin, corresponding to the production
of 112.82 µg/mL of HMG-CoA reductase inhibitors (simvastatin), in the medium
containing 4.5g of cassava peels, temperature at 28° C, pH 6.5, in 7 days of incubation.
The production of HMG-CoA reductase inhibitors (simvastatin) occurs in the limiting
condition of nitrogen and carbon sources, as important in the bioprocess. The results
suggest that cassava husks can be used as a renewable and economical source in the
production of HMG-CoA reductase inhibitors similar simvastatin by A. terreus UCP
0076, favoring the principles of circular bioeconomy and environmental sustainability.