Artículos de revistas
Lack of serological and molecular evidences of Zika virus circulation in non-human primates in three states from Brazil
Fecha
2022-01-01Registro en:
Memorias Do Instituto Oswaldo Cruz. Rio De Janeiro, Rj: Fundaco Oswaldo Cruz, v. 117, 6 p., 2022.
0074-0276
10.1590/0074-02760220012
WOS:000854986400001
Autor
Universidade Estadual Paulista (UNESP)
Univ Fed Parana
Universidade de São Paulo (USP)
Univ Fed Campina Grande
Refugio Biol Bela Vista
Zool Bosque Guarani
Zool Municipal Quinzinho Barros
Univ Sorocaba
Univ Fed Paraiba
Univ Fed Integracao Latino Amer
Univ Texas Med Branch
Universidade Federal de Mato Grosso do Sul (UFMS)
Institución
Resumen
BACKGROUND Zika virus (ZIKV) was discovered in 1947 with the virus isolation from Rhesus monkey (Macaca mulatta) in Uganda forest, Africa. Old World Primates are involved in a sylvatic cycle of maintenance of this arbovirus, however a limited knowledge about the role of New World primates in ZIKV transmission cycles has been established. OBJECTIVE This work aimed to investigate the presence of enzootic circulation of ZIKV in New World Primates from three Brazilian states: Sao Paulo, Paraiba, and Parana. METHODS We analyzed 100 non-human primate samples collected in 2018 and 2020 from free-ranging and captive environments from Sao Paulo (six municipalities belonging to Sorocaba region), Paraiba (Joao Pessoa municipality), and Parana (Foz do Iguacu municipality) using reverse transcriptase quantitative polymerase reaction (RT-qPCR) assays, indirect enzyme-linked immunosorbent assay (ELISA), and plaque reduction neutralization test (PRNT). FINDINGS All samples (n = 141) tested negative for the presence of ZIKV genome from tissue and blood samples. In addition, all sera (n = 58) from Foz do Iguacu' non-human primates (NHPs) were negative in serological assays. MAIN CONCLUSION No evidence of ZIKV circulation (molecular and serological) was found in neotropical primates. In addition, the absence of antibodies against ZIKV suggests the absence of previous viral exposure of NHPs from Foz do Iguacu-PR.