Artículos de revistas
Effects of (−)-6,6′-dinitrohinokinin on adult worms of Schistosoma mansoni: A proteomic analyses
Fecha
2016-05-01Registro en:
Revista Brasileira de Farmacognosia, v. 26, n. 3, p. 334-341, 2016.
1981-528X
10.1016/j.bjp.2016.02.001
2-s2.0-84975034810
Autor
Universidade de Franca
Universidade de São Paulo (USP)
Universidade Federal de Uberlândia (UFU)
Universidade do Estado de São Paulo
Universidade de Juiz de Fora
Institución
Resumen
Schistosomiasis, a chronic disease that affects million people worldwide, is caused by trematodeflukes of the genus Schistosoma. The lack of an anti-schistosomiasis vaccine and massive monother-apy with praziquantel reinforces the need for search and development of new therapeutic drugs.Recently, we demonstrated that the essential oil of Piper cubeba L., Piperaceae, and their derivative diben-zylbutyrolactolic (−)-6,6′-dinitrohinokinin, presents in vitro and in vivo activities against Schistosomamansoni. Here, we identified changes in the protein expression after exposure to dibenzylbutyrolac-tolic (−)-6,6′-dinitrohinokinin. We applied two-dimensional gel electrophoresis (2-DE) to S. mansonisoluble protein extracts and observed at least 38 spots to be affected by dibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin. We further identified 25 differentially expressed proteins by mass spectrometry.Enrichment for biological processes and predictive analyses of protein-protein interactions suggest thatdibenzylbutyrolactolic (−)-6,6′-dinitrohinokinin targets proteins involved mainly in metabolic processes,especially carbohydrate metabolism. In summary, this study provides an interesting approach to under-stand the anti-parasitic activity of semi-synthetic (−)-6,6′-dinitrohinokinin a derivative compound fromlignan and for the development of new therapy strategies.