Epicormic bud protection traits vary along a latitudinal gradient in a neotropical savanna

Abstract

Regrowth via production of epicormic shoots is an important strategy for many woody plants after environmental disturbances such as fire, drought, and herbivory. Populations spreading across a broad latitudinal gradient offer opportunities to investigate if essential traits vary with heterogenous environmental conditions, such as in savanna ecosystems. This information can help us predict plant responses to climate change. Here, we evaluated if epicormic bud protection traits varied among populations of three focal savanna species (Miconia albicans, Solanum lycocarpum, and Zeyheria montana) that have a wide distribution and grow under variable climatic conditions. We randomly sampled 225 individuals over five spatially independent sites (7°, 10°, 15°, 18°, and 24° S) in Brazil, totaling 15 individuals per species per area. We analyzed anatomical transverse sections of five buds per species per area to assess the relative area occupied by crystal and phenolic idioblasts, the thickness of the trichome boundary layer, and to test if these traits were associated with climatic conditions. The buds were protected by cataphylls and composed of a variable number of undeveloped leaves enveloping the shoot apex. For M. albicans, we found an association between maximum temperature and both phenolic idioblasts and trichome boundary layer, but no association with crystal idioblasts. In S. lycocarpum, only the trichome boundary layer was associated with maximum temperature plus high radiation. Z. montana showed no variation. Combination of two or more traits can lead to the development of adaptative strategies to different climatic conditions. We present for the first time an analysis of epicormic bud traits in plant populations occurring in an extensive latitudinal gradient and shed light on how maximum temperature is associated with these traits, contributing to a better understanding of plant resprouting capabilities in widespread savanna plant species.