Artículos de revistas
Influence of hepes on in vitro maturation of canine oocytes in different stages of the estrous cycle
Influência do hepes na maturação in vitro de oócitos caninos obtidos de cadelas em diestro e anestro
Fecha
2019-01-01Registro en:
Ciencia Animal Brasileira, v. 20.
1809-6891
1518-2797
10.1590/1809-6891v20e-53372
S1809-68912019000100335
2-s2.0-85082975524
S1809-68912019000100335.pdf
Autor
Universidade Estadual Paulista (Unesp)
Universidade Estadual do Ceará
Institución
Resumen
Unlike other domestic animals, in vitro maturation (IVM) of canine oocytes still has limited success. The aim of this study was to evaluate the effect of HEPES on the in vitro maturation of dog oocytes in anestrus and diestrus. Ovaries were collected, aseptically isolated and transported refrigerated at a temperature of 4°C. Cumulus-oocyte complexe (COCs) from the two phases of estrous cycle were subjected to two treatments: medium TCM-199 with addition of 25 mM HEPES (TG) and medium without supplementation (CG). After 72 h of maturation, COCs were denuded, fixed and stained with Hoechst 33342 to assess nuclear maturation. The oocytes obtained from the anestrus and diestrus phases of the TG showed a higher frequency of oocytes in metaphase II (M-II) (p < 0.01) stage. Comparing the different reproductive status, it was observed that the oocytes obtained from the diestrus phase presented higher rates of GVBD (germinal vesicle breakdown) and M-II. Our results showed that HEPES preserves the viability and oocyte morphology essential for the acquisition of meiotic competence, increasing the M-II rates and that the oocytes obtained from the diestrus phase are better able to complete oocyte maturation.