Artículos de revistas
SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
Fecha
2019-04-01Registro en:
Brazilian Journal Of Chemical Engineering. Sao Paulo: Brazilian Soc Chemical Eng, v. 36, n. 2, p. 657-668, 2019.
0104-6632
10.1590/0104-6632.20190362s20180572
S0104-66322019000200657
WOS:000488278700005
S0104-66322019000200657.pdf
9620122455708223
0000-0003-4069-509X
Autor
Univ Fed Alfenas
Universidade Estadual Paulista (Unesp)
Inst Pesquisas Tecnol
Univ Minho
Institución
Resumen
Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work aimed to evaluate the cellular growth, production and characterization of mycelial and extracellular FTases by Aspergillus oryzae IPT-301. Experimental design showed that the extracellular FTase performance was optimized (high transfructosylation activity and low hydrolytic activity) for reaction pH 5.5-6.75 and temperature of 45-50 degrees C and was fitted by the Michaelis-Menten model, while the mycelial FTase showed better performance at pH below 6.5 and temperature above 46 degrees C and was better fitted by the Hill model. The results obtained showed that the fungus represents a promising source for FOS production on a laboratorial scale.