es | en | pt | fr
    • Presentation
    • Countries
    • Institutions
    • Get involved
        JavaScript is disabled for your browser. Some features of this site may not work without it.
        View Item 
        •   Home
        • Brasil
        • Universidades
        • Universidade Estadual de Campinas (Brasil)
        • View Item
        •   Home
        • Brasil
        • Universidades
        • Universidade Estadual de Campinas (Brasil)
        • View Item

        CREB is involved in the regulation of POMC processing enzymes andα‐MSH production in the hypothalamus of mice

        Registration in:
        https://doi.org/10.25824/redu/WMNTS8
        https://repositorioslatinoamericanos.uchile.cl/handle/2250/5363158
        Author
        Zanesco, Ariane Maria
        Institutions
        • Universidade Estadual de Campinas (Brasil)
        Abstract
        The hypothalamic proopiomelanocortin (POMC) neurons act as first order sensors of systemic energy stores, providing signals that regulate caloric intake and energy expenditure. In experimental obesity, dietary saturated fatty acids affect POMC endopeptidases, resulting in the abnormal production of the neurotransmitters a-melanocyte stimulating hormone (a-MSH) and b-endorphin, thus impacting energy balance. The cAMP response element-binding protein (CREB) is one of the transcription factors that control the expression of POMC endopeptidases; however, it was previously unknown if dietary fats could affect CREB and consequently the expression of POMC endopeptidases. Here, we used single-cell RNA sequencing analysis, PCR, immunoblot, ELISA and immunofluorescence histological assays to determine the impact of a high-fat diet on the expression and function of hypothalamic CREB and its impact on the melanocortinergic system. The results indicate that CREB is expressed in arcuate nucleus POMC neurons and is activated shortly after the introduction of a high-fat diet. The inhibition of hypothalamic CREB using a short-harpin RNA lentiviral vector resulted in increased diet-induced body mass gain and reduced energy expenditure. This was accompanied by reduced expression of the POMC endopeptidases Pcsk2, Prcp and Cpe and by the loss of the high-fat diet-induced effect of a-MSH inhibition. This study provides the first evidence for the involvement of CREB in the abnormal regulation of the hypothalamic POMC endopeptidase system in experimental obesity
        Subjects
        Medicine, Health and Life Sciences
        Proopiomelanocortin
        Obesity
        Diet
        Saturated fatty acid
        CREB

        Show full item record


        Red de Repositorios Latinoamericanos
        + of 8.000.000
        Available publications
        500 Participating institutions
        Dirección de Servicios de Información y Bibliotecas (SISIB)
        Universidad de Chile
        Membership Login
        Featured collections
        • Latin American Theses
        • Argentinean Theses
        • Chilean Theses
        • Peruvian Theses
        Latest collections added
        • Argentina
        • Brazil
        • Colombia
        • México
        Dirección de Servicios de Información y Bibliotecas (SISIB)
        Universidad de Chile
        Red de Repositorios Latinoamericanos | 2006-2018
         

        EXPLORE BY

        Institutions
        Date2011 - 20202001 - 20101951 - 20001901 - 19501800 - 1900

        Browse in Repository Network

        Countries >
        Document type >
        Publication date >
        Institutions >

        Red de Repositorios Latinoamericanos
        + of 8.000.000
        Available publications
        500 Participating institutions
        Dirección de Servicios de Información y Bibliotecas (SISIB)
        Universidad de Chile
        Membership Login
        Featured collections
        • Latin American Theses
        • Argentinean Theses
        • Chilean Theses
        • Peruvian Theses
        Latest collections added
        • Argentina
        • Brazil
        • Colombia
        • México
        Dirección de Servicios de Información y Bibliotecas (SISIB)
        Universidad de Chile
        Red de Repositorios Latinoamericanos | 2006-2018