Eighteen steroid-related compounds were found to be chemical constituents for a steroidal fraction obtained from the fruiting body of the edible mushroom Laetiporus sulphureus on the basis of GC-MS analyses. Compounds were found to be mostly associated to the ergostane and lanostane moieties. Lanost-8,25-dien- 3β-ol 3 (8.8%), lanost-7,9(11),24-trien-3β,15α-diol (polycarpol) 11 (12.7%), 15-norlanost-9(11)-en-3β,14,15-triol 13 (15.3%), and 3β,15α-dihydroxy-24-methylenelanost-8-en-21-oic acid (sulphurenic acid) 18 (15.4%), were found to be the main compounds.