The aim of this paper was to study the in vitro antimicrobial activity of royal jelly obtained from supplemented honey bees against the microorganism Staphylococcus aureus using the disk diffusion method. The royal jelly was obtained from Apis mellifera africanized honey bees fed with complete supplement constituted of palm oil plus linseed oil, palm oil alone, linseed oil alone and with no supplementation. Samples obtained from the different supplementations were applied to paper disks in the concentration range 0 -100%.  All the samples of royal jelly were able to inhibit the growth of S. aureus in a dose-dependent manner. There was no difference between the antimicrobial activity of the different samples of royal jelly obtained from supplemented or not supplemented bees. DOI: http://dx.doi.org/10.14685/rebrapa.v1i1.3