dc.creatorMárquez Rodríguez, Lorenzo
dc.creatorHernández Arias, Adrián
dc.creatorCarvalho, Edison S.M
dc.creatorMorales, Gabriel Alejandro
dc.creatorDantagnan Dantagnan, Patricio
dc.creatorDíaz López, Manuel
dc.creatorMoyano López, Francisco
dc.date2019
dc.date2022-10-21T16:25:38Z
dc.date2022-10-21T16:25:38Z
dc.date.accessioned2022-12-06T13:19:41Z
dc.date.available2022-12-06T13:19:41Z
dc.identifierLatin American Journal of Aquatic Research, Vol. 47, N° 5, 860-864, 2019
dc.identifier10.3856/vol47-issue5-fulltext-16
dc.identifierhttps://repositoriodigital.uct.cl/handle/10925/4811
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5238365
dc.descriptionThe activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.
dc.formatPDF
dc.formatapplication/pdf
dc.languageen
dc.publisherPontificia Universidad Católica de Valparaíso
dc.rightsObra bajo licencia Creative Commons Atribución-NoComercial 4.0 Internacional
dc.sourceLatin American Journal of Aquatic Research (Valparaíso)
dc.subjectOncorhynchus kisutch
dc.subjectCoho salmon
dc.subjectGastric proteases
dc.subjectEnzymatic activity
dc.subjectpH
dc.subjectIonic strength
dc.subjectSalmón plateado
dc.subjectProteasas gástricas
dc.subjectActividad enzimatica
dc.subjectFuerza iónica
dc.titleEffects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
dc.typeArtículo de Revista
dc.coverageChile


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