| dc.contributor | Gómez Kosky, R., Instituto de Biotecnología de Las Plantas, Universidad Central de Las Villas, Carretera a Camajuani km 5.5, Santa Clara, Villa Clara, Cuba; De Feria Silva, M., Instituto de Biotecnología de Las Plantas, Universidad Central de Las Villas, Carretera a Camajuani km 5.5, Santa Clara, Villa Clara, Cuba; Posada Pérez, L., Instituto de Biotecnología de Las Plantas, Universidad Central de Las Villas, Carretera a Camajuani km 5.5, Santa Clara, Villa Clara, Cuba; Gilliard, T., Ministry of Agriculture, St. Lucia, Australia; Bernal Martínez, F., Universidad de Guadalajara, Mexico; Reyes Vega, M., Instituto de Biotecnología de Las Plantas, Universidad Central de Las Villas, Carretera a Camajuani km 5.5, Santa Clara, Villa Clara, Cuba; Chávez Milian, M., Instituto de Biotecnología de Las Plantas, Universidad Central de Las Villas, Carretera a Camajuani km 5.5, Santa Clara, Villa Clara, Cuba; Quiala Mendoza, E., Instituto de Biotecnología de Las Plantas, Universidad Central de Las Villas, Carretera a Camajuani km 5.5, Santa Clara, Villa Clara, Cuba | |
| dc.description.abstract | The development of somatic embryos in liquid culture medium has a number of advantages for large-scale propagation of plants. This paper describes an improved system for the mass propagation via somatic embryogenesis of the banana hybrid cultivar FHIA-18 (AAAB). Explants from immature male flowers were used to form high frequency embryogenic tissue, this tissue was then used to establish embryogenic cell suspensions in a basic MS medium plus 1.0 mg 1 -1 biotin, 100 mg 1 -1 glutamine, 100 mg 1 -1 malt extract (Sigma), 1.0 mg 1 -1 2,4-D and 45 g 1 -1 sucrose. Secondary multiplication of somatic embryos was achieved in liquid media in rotary shaker and in bioreactors. The number of embryos per litre obtained with 80.0% DO 2 and effects of pH were also studied. A high regeneration percentage of plants were obtained (89.3%) in only 1 month of culture, somatic embryos were then placed to germinate in temporary immersion systems and field testing of somaclonal variation. | |
