Characterization of Plasmid-Mediated Quinolone Resistance (PMQR) Genes in Extended-Spectrum ?-Lactamase-Producing Enterobacteriaceae Pediatric Clinical Isolates in Mexico

Abstract

The present research contains the geographical references detailed by municipal including climatic characteristics, marginal areas, optimal and suboptimal areas where Jamaica as an alternative crop represents a viable option for the maintainable development of those regions with climatic limitations for other crops. Based on the reference data it is considered 125 humidity days as the optimal to develop the crop. In Jalisco 900,000 ha has been reported whit this condition within the nine climatic zones existing. " 2009 Asian Network for Scientific Information.",,,,,,"10.3923/ajps.2009.92.101",,,"http://hdl.handle.net/20.500.12104/40004","http://www.scopus.com/inward/record.url?eid=2-s2.0-67149094768&partnerID=40&md5=408e8aa09266da75a58e857bd9f325fe",,,,,,"2",,"Asian Journal of Plant Sciences",,"92

101",,"8",,"Scopus",,,,,,"Global positioning system; Jalisco-Mexico; Jamaica; Weather maps",,,,,,"Characterization of the climatic rankins for jamaica (Hibiscus sabdariffa L.) Crop in Jalisco, Mexico",,"Article" "41790","123456789/35008",,"Carbonell, C.S., Facultadde Ciencias, Universidadde la República, Uruguay; Rowell, C.H.F., Zoologisches Institut der Universityt Basel, Switzerland; Abentos-Pereira, A., Centro Universitario Los Lagos, Universidad de Guadalajara, Jalisco, Mexico; Porras, M.F., Universidad Nacional de Colombia, Instituto de Ciencias Biológicas",,"Carbonell, C.S.

Rowell, C.H.F.

Abentos-Pereira, A.

Porras, M.F.",,"2007",,"[No abstract available]",,,,,,,,,"http://hdl.handle.net/20.500.12104/40011","http://www.scopus.com/inward/record.url?eid=2-s2.0-34948909784&partnerID=40&md5=e03990cde2320cf6b1a9a9d565dab395",,,,,,"1594",,"Zootaxa",,"39

59",,,,"Scopus

WOS",,,,,,,,,,,,"Checklist of orthoptera caelifera from Colombia",,"Article" "41780","123456789/35008",,"Silva-Sánchez, J., Instituto Nacional de Salud Pública, Centro de Investigación sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico; Cruz-Trujillo, E., Instituto Nacional de Salud Pública, Centro de Investigación sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico; Barrios, H., Instituto Nacional de Salud Pública, Centro de Investigación sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico; Reyna-Flores, F., Instituto Nacional de Salud Pública, Centro de Investigación sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico; Sánchez-Pérez, A., Instituto Nacional de Salud Pública, Centro de Investigación sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico; Garza-Ramos, U., Instituto Nacional de Salud Pública, Centro de Investigación sobre Enfermedades Infecciosas (CISEI), Departamento de Diagnóstico Epidemiológico, Cuernavaca, Morelos, Mexico; Morfin-Otero, R., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Unidad de Investigación en Epidemiología Hospitalaria, Hospital de Pediatría, Centro Medico Nacional Siglo XXI, Ciudad de México, Mexico; Rodríguez-Noriega, E., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Unidad de Investigación en Epidemiología Hospitalaria, Hospital de Pediatría, Centro Medico Nacional Siglo XXI, Ciudad de México, Mexico; Novales, G.M., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Departamento de Bacteriología, Hospital General de Tapachula Chiapas, Tapachula, Chiapas, Mexico; Solórzano, F., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Departamento de Bacteriología, Hospital General de Tapachula Chiapas, Tapachula, Chiapas, Mexico; Díaz, R., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Departamento de Bacteriología, Hospital General de Tapachula Chiapas, Tapachula, Chiapas, Mexico; Ramírez, F., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Departamento de Ens?anza, Investigación y Calidad, Hospital del Niño, Dr. Rodolfo Nieto Padrón y Universidad Juarez Autónoma de Tabasco, Villahermosa, Tabasco, Mexico; Gil, L.P., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Q.B.P. Edgardo Salvador Acevedo Casarrubias, Servicio de Gastroenterología, Hosp. Universitario Dr. José Eleuterio González, Universidad Autónoma de Nuevo León, Nuevo León, Mexico; Garza, E., Hospital Civil de Guadalajara Fray Antonio Alcalde, Instituto de Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico",,"Silva-Sanchez, J.

Cruz-Trujillo, E.

Barrios, H.

Reyna-Flores, F.

Sanchez-Perez, A.

Garza-Ramos, U.

Morfin-Otero, R.

Rodriguez-Noriega, E.

Novales, G.M.

Solorzano, F.

Diaz, R.

Ramirez, F.

Gil, L.P.

Garza, E.",,"2013",,"This work describes the characterization of plasmid-mediated quinolone-resistance (PMQR) genes from a multicenter study of ESBL-producing Enterobacteriaceae pediatric clinical isolates in Mexico. The PMQR gene-positive isolates were characterized with respect to ESBLs, and mutations in the GyrA and ParC proteins were determined. The phylogenetic relationship was established by PFGE and the transfer of PMQR genes was determined by mating assays. The prevalence of the PMQR genes was 32.1%, and the rate of qnr-positive isolates was 15.1%; 93.3% of the latter were qnrB and 6.4% were qnrA1. The distribution of isolates in terms of bacterial species was as follows: 23.5% (4/17) corresponded to E. cloacae, 13.7% (7/51) to K. pneumoniae, and 13.6% (6/44) to E. coli. In addition, the prevalence of aac(6')-Ib-cr and qepA was 15.1% and 1.7%, respectively. The molecular characteristics of qnr- and qepA-positive isolates pointed to extended-spectrum ?-lactamase (ESBL) CTX-M-15 as the most prevalent one (70.5%), and to SHV-12 in the case of aac(6')-Ib-cr-positive isolates. GyrA mutations at codons Ser-83 and Asp-87, and ParC mutations at codons Ser-80 were observed in 41.1% and 35.2% of the qnr-positive isolates, respectively. The analysis of the transconjugants revealed a co-transmission of blaCTX-M-15 with the qnrB alleles. In general, the prevalence of PMQR genes (qnr and aac(6')-Ib-cr) presented in this work was much lower in the pediatric isolates, in comparison to the adult isolates in Mexico. Also, ESBL CTX-M-15 was the main ESBL identified in the pediatric isolates, whereas in the adult ones, ESBLs corresponded to the CTX-M and the SHV families. In comparison with other studies, among the PMQR-genes identified in this study, the qnrB-alleles and the aac(6')-Ib-cr gene were the most prevalent, whereas the qnrS1, qnrA1 and qnrB-like alleles were the most prevalent in China and Uruguay. " 2013 Silva-Sanchez et al.