Instrumentation for a plasma needle applied to E. coli bacteria elimination

Abstract

Background and Purpose: Renal calculi may contain bacteria that can remain active inside the stone and produce bacteremia and/or endotoxemia after lithotripsy. Urinary tract infection associated with urinary stones represents high morbidity. The purpose of this research was to use novel artificial struvite stones inoculated with living bacteria and to study the effect of four different intracorporeal lithotripters on bacterial inactivation after in vitro lithotripsy. Materials and Methods: Two types of artificial kidney stone models (calcium sulphate and mixed struvite-calcium sulphate) were manufactured and infected with Proteus mirabilis. Stones were fractured using either electrohydraulic, laser, ultrasonic, or pneumatic lithotripters. Bacterial viability was determined before and after the lithotripsy. Results: Bacterial inactivation was not affected by the stone matrix; ie, calcium or struvite. The four tested lithotripters were almost equally efficient at reducing the viability of P mirabilis in both the low and the high energy setting. Conclusions: We were able to obtain novel artificial struvite stones infected with bacteria. Intracorporeal lithotripters are efficient at reducing the viability of P mirabilis in vitro. Tested stone materials play a minor role regarding inactivation. Whether the bactericidal effect reported is desirable or not is still to be answered, because the presence of endotoxin from cell lysis may increase the risk of urosepsis. " Copyright 2009, Mary Ann Liebert, Inc. 2009.",,,,,,"10.1089/end.2008.0264",,,"http://hdl.handle.net/20.500.12104/42299","http://www.scopus.com/inward/record.url?eid=2-s2.0-65549105457&partnerID=40&md5=c79bc13cd4abc6724df051d1336e57ee",,,,,,"3",,"Journal of Endourology",,"519

522",,"23",,"Scopus

WOS",,,,,,,,,,,,"Interaction of intracorporeal lithotripters with proteus mirabilis inoculated inside artificial calcium and struvite stones",,"Article" "44071","123456789/35008",,"García-Contreras, A.A., Instituto de Nutrición Humana, Universidad de Guadalajara, Mexico; Vásquez-Garibay, E.M., Instituto de Nutrición Humana, Universidad de Guadalajara, Mexico, Hospital Civil de Guadalajara Dr. Juan I. Menchaca, Mexico; Romero-Velarde, E., Instituto de Nutrición Humana, Universidad de Guadalajara, Mexico, Hospital Civil de Guadalajara Dr. Juan I. Menchaca, Mexico; Ibarra-Gutiérrez, A.I., Hospital Civil de Guadalajara Dr. Juan I. Menchaca, Mexico; Troyo-Sanromán, R., Instituto de Nutrición Humana, Universidad de Guadalajara, Mexico; Sandoval-Montes, I.E., Instituto de Nutrición Humana, Universidad de Guadalajara, Mexico",,"García-Contreras, A.A.

Vasquez-Garibay, E.M.

Romero-Velarde, E.

Ibarra-Gutierrez, A.I.

Troyo-Sanroman, R.

Sandoval-Montes, I.E.",,"2014",,"Objective: To demonstrate that a nutritional support intervention, via naso-enteral tube-feeding or gastrostomy, has a significant impact on the nutritional status and body composition in severely malnourished children with cerebral palsy spastic quadriplegia Methods: Thirteen patients with moderate/severe malnutrition and cerebral palsy spastic quadriplegia who were fed via naso-enteral tube-feeding or gastrostomy were included in a cohort study. Anthropometric measurements and estimated body composition by bioelectric impedance analysis were obtained. ANOVA and Wilcoxon tests were used. Results: During the four weeks of nutritional recovery, an average weight increase of 2700 g was achieved. There were significant increases in anthropometric indicators, including BMI and weight/length (p < 0.01). The increase in arm fat area was significantly higher than the increase in arm muscle area (104.5 vs 17.5%). Conclusion: Intensive nutritional support for four weeks had a significant effect on the nutritional status and body composition of severe and moderately malnourished children with cerebral palsy spastic quadriplegia.",,,,,,"10.3305/nh.2014.29.4.7247",,,"http://hdl.handle.net/20.500.12104/42292","http://www.scopus.com/inward/record.url?eid=2-s2.0-84897932149&partnerID=40&md5=e42371cf9966621cc81e210832d24659

http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=medl&AN=24679025",,,,,,"4",,"Nutricion Hospitalaria",,"838

843",,"29",,"Scopus

MEDLINE

WOS",,,,"Index Medicus;Body Composition;Cerebral Palsy/co [Complications];Cerebral Palsy/th [Therapy];Child;Enteral Nutrition;Female;Humans;Male;Malnutrition/co [Complications];Malnutrition/th [Therapy];Nutritional Status;Nutritional Support/mt [Methods];Quadriplegia/co [Complications]",,"Body composition; Cerebral palsy; Nutritional recovery; Severe malnutrition",,,,,,"Intensive nutritional support improves the nutritional status and body composition in severely malnourished children with cerebral palsy [El apoyo nutricio intensivo mejora el estado nutricio y la composición corporal en niños gravemente desnutridos con parálisis cerebral]",,"Article" "44063","123456789/35008",,"Peña-Eguiluz, R., Instituto Nacional de Investigaciones Nucleares, Laboratorio de Física de Plasmas, AP 18-1027, 11801 México D.F., Mexico; Pérez-Martínez, J.A., Instituto Tecnológico de Toluca, AP 890, Toluca, Estado de México, Mexico; Solís-Pacheco, J., Universidad de Guadalajara, CUCEI, Departamento de Farmacobiología, Blvd. Marcelino Barragán No. 1421 esq., Calz. Olímpica, Guadalajara, Mexico; Aguilar-Uscanga, B., Universidad de Guadalajara, CUCEI, Departamento de Farmacobiología, Blvd. Marcelino Barragán No. 1421 esq., Calz. Olímpica, Guadalajara, Mexico; López-Callejas, R., Instituto Nacional de Investigaciones Nucleares, Laboratorio de Física de Plasmas, AP 18-1027, 11801 México D.F., Mexico, Instituto Tecnológico de Toluca, AP 890, Toluca, Estado de México, Mexico; Mercado-Cabrera, A., Instituto Nacional de Investigaciones Nucleares, Laboratorio de Física de Plasmas, AP 18-1027, 11801 México D.F., Mexico; Valencia-Alvarado, R., Instituto Nacional de Investigaciones Nucleares, Laboratorio de Física de Plasmas, AP 18-1027, 11801 México D.F., Mexico; Muñoz-Castro, A.E., Instituto Nacional de Investigaciones Nucleares, Laboratorio de Física de Plasmas, AP 18-1027, 11801 México D.F., Mexico; Barocio, S.R., Instituto Nacional de Investigaciones Nucleares, Laboratorio de Física de Plasmas, AP 18-1027, 11801 México D.F., Mexico; De La Piedad Beneitez, A., Instituto Tecnológico de Toluca, AP 890, Toluca, Estado de México, Mexico",,"Pena-Eguiluz, R.

Perez-Martinez, J.A.

Solis-Pacheco, J.

Aguilar-Uscanga, B.

Lopez-Callejas, R.

Mercado-Cabrera, A.

Valencia-Alvarado, R.

Munoz-Castro, A.E.

Barocio, S.R.

De La Piedad Beneitez, A.",,"2010",,"Microplasmas are nowadays a powerful tool with multiple practical applications. The performance of a specific instrumentation for a plasma needle capable of producing non-thermal plasmas and a DBD reactor able to produce atmospheric pressure plasmas, both of them designed and already constructed, is reported. These devices operate at 13.56 MHz and are driven by a specifically built radio frequency (RF) resonant converter. The reactors, which operate at atmospheric pressure in a He-air gas mixture at a 1.5 SLPM flow, have been successfully applied to eliminate E. coli bacteria. In the needle case, bacterial samples were submitted typically to a 500 V peak voltage plasma discharge for 120 s. In the DBD treatment, the samples were processed with typical 750 V peak voltage plasma discharges for 80 s. The sample pH was used as a criterion to measure the effectiveness of the plasma treatment, in such a way that the return to the basal pH value after the treatment can be assumed as the validation of the complete bacterial elimination. " 2009 EDP Sciences.