| dc.contributor | Kuzmenko, V., Wallenberg Wood Science Center, Kemivägen 4, SE-412 96 Gothenburg, Sweden, Department of Microtechnology and Nanoscience, Chalmers University of Technology, Kemivägen 9, SE-412 96 Gothenburg, Sweden; Hägg, D., Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 4, SE-412 96 Gothenburg, Sweden; Toriz, G., Wallenberg Wood Science Center, Kemivägen 4, SE-412 96 Gothenburg, Sweden, Department of Wood, Cellulose and Paper Research, University of Guadalajara, Juárez 976, 44100 Guadalajara, Mexico; Gatenholm, P., Wallenberg Wood Science Center, Kemivägen 4, SE-412 96 Gothenburg, Sweden, Department of Chemical and Biological Engineering, Chalmers University of Technology, Kemivägen 4, SE-412 96 Gothenburg, Sweden | |
| dc.description.abstract | An in situ forming spruce xylan-based hydrogel was synthesized in two steps with the intended use of cell encapsulation and in vivo delivery. First, bioconjugate was obtained through the reaction of glucuronic acid groups from xylan backbone with tyramine (TA). After that, the gelation process was enabled by enzymatic crosslinking of the phenol-containing TA-xylan conjugate. Exhibiting an exponential increase in the storage modulus, a 3D gel network was formed in about 20 s. The designed gel showed extensive swelling and retained its mechanical integrity for more than two months. Mesenchymal stem cells were encapsulated in the hydrogel and cultured for one week. The cells retained their adipogenic differentiation capacity inside the gel, as verified by lipid accumulation. From these facts, we conclude that spruce xylan is a promising precursor for in situ forming hydrogels and should be evaluated further for tissue engineering purposes. © 2013 Elsevier Ltd. | |
