Genotoxic effect of chronic exposure to DDT on lymphocytes, oral mucosa and breast cells of female rats

Abstract

The objective of this study was to assess and quantify the degree to which interaction between occupational driving and lifting tasks is a risk factor in lumbar spondyloarthrosis etiology. A case-control study was performed with 231 workers, 18-55 years old, insured by the Mexican Social Security Institute (IMSS, according to its designation in Spanish). A multivariate analysis using conditional logistical regression showed that driving tasks, when combined with lifting tasks, are associated with this illness (OR = 7.3; 95% CI 1.7-31.4). Occupational driving as it interacted with daily lifting frequency resulted in a greater risk (OR = 10.4; 95% CI 2.0-52.5). No exposure-response relationship was found with daily hours spent working as a driver. The attributable risk for driving tasks was 0.86, suggesting that 86% of lumbar spondyloarthrosis could be decreased if risk factors were reduced through ergonomic redesign of the workplace and Manual Materials Handling (MMH) tasks, along with development of educational programs. " 2008 IOS Press. All rights reserved.",,,,,,,,,"http://hdl.handle.net/20.500.12104/43288","http://www.scopus.com/inward/record.url?eid=2-s2.0-58149343800&partnerID=40&md5=8d62fbcd037211609a2b7f40f93196bb

http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&D=med5&AN=19127009",,,,,,"4",,"Work",,"387

396",,"31",,"Scopus

MEDLINE

WOS",,,,"Index Medicus;Adolescent;Adult;Automobile Driving/sn [Statistics & Numerical Data];Case-Control Studies;Female;Human Engineering;Humans;Lifting/ae [Adverse Effects];Logistic Models;Low Back Pain/cl [Classification];Low Back Pain/et [Etiology];Low Back Pain/pc [Prevention & Control];Male;Mexico/ep [Epidemiology];Middle Aged;Occupational Diseases/cl [Classification];Occupational Diseases/et [Etiology];Occupational Diseases/pc [Prevention & Control];Risk Factors;Young Adult",,"Driving; Lifting; Low back pain; Occupational disease/prevention & control; Risk factors",,,,,,"Occupational driving as a risk factor in low back pain: A case-control study in a Mexican population",,"Article" "43507","123456789/35008",,"Canales-Aguirre, A., Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of Jalisco, A.C. (CIATEJ), Normalistas 800, Guadalajara, 44270, Mexico; Padilla-Camberos, E., Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of Jalisco, A.C. (CIATEJ), Normalistas 800, Guadalajara, 44270, Mexico; Gómez-Pinedo, U., Laboratory of Regenerative Medicine, Neuroscience Institute, Hospital Clinico San Carlos, Madrid, 28040, Spain; Salado-Ponce, H., Unit of Medical and Pharmaceutical Biotechnology, Center for Research and Assistance in Technology and Design of Jalisco, A.C. (CIATEJ), Normalistas 800, Guadalajara, 44270, Mexico; Feria-Velasco, A., Biological Sciences Department, Biological and Agricultural Sciences Center, University of Guadalajara, Carr. a Nogales Km 15.5, Zapopan, 45110, Mexico, BiosMedica Cancer Research Institute, Alcatraces 72, Jardin Real, Zapopan, 45136, Mexico; de Celis, R., BiosMedica Cancer Research Institute, Alcatraces 72, Jardin Real, Zapopan, 45136, Mexico, Environmental Immunology Laboratory, Western Biomedical Research Center of the Mexican, Institute for Social Security, Sierra Mojada 800, Colonia Independencia, S.L., Guadalajara, 44340, Mexico",,"Canales-Aguirre, A.

Padilla-Camberos, E.

Gomez-Pinedo, U.

Salado-Ponce, H.

Feria-Velasco, A.

de Celis, R.",,"2011",,"The genotoxicity of some environmental contaminants may affect human health directly by damaging genetic material and thus plays an important role in cancer development. Xenoestrogens are one kind of environmental pollutants that may alter hormonal routes or directly affect DNA. The number of available biomarkers used to assess genetic risk and cancer is very extensive. The present study evaluated genotoxicity produced by the pesticide DDT on systemic and mammary gland cells obtained from adult female Wistar rats. Oral mucosa cells micronuclei were assessed; the comet assay in peripheral blood-isolated lymphocytes and mammary epithelial cells was also carried out. Additionally, oxidative stress was studied in mammary tissue through a lipid peroxidation assay. Our data showed an increase in lipid peroxidation, product of an increase in free oxygen radical levels, which leads to an oxidative stress status. Our results suggest that DDT is genotoxic, not only for lymphocytes but also to mammary epithelial cells. " 2011 by the authors; licensee MDPI, Basel, Switzerland.