| dc.contributor | Nuño-Arana, I., Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCiénega-UdeG), Ocotlán, Jalisco, Mexico; Sahagún-Núñez, V.R., Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, Mexico; Muñoz-Valle, J.F., Grupo de Inmunogenética Funcional, Departamento de Biología Molecular y Genómica, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara (CUCS-UdeG), Guadalajara, Jalisco, Mexico; Sandoval, L., División de Genética, Centro de Investigación Biomédica de Occidente (CIBO-IMSS), Guadalajara, Jalisco, Mexico; Pinto-Escalante, D., Laboratorio de Genética, Centro Universitario de los Lagos, Universidad de Guadalajara (CULagos-UdeG), Lagos de Moreno, Jalisco, Mexico; Páez-Riberos, L.A., Centro de Investigacioines Regionales Hideyo Noguchi, Universidad Autónoma de Yucatán (UADY), Mérida, Yucatán, Mexico; Lazalde, B., Unidad de Investigación Biomédica, IMSS, Durango, Mexico; Maldonado-González, M., Servicio de Biología Molecular en Medicina, Antiguo Hospital Civil de Guadalajara, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Rangel-Villalobos, H., Instituto de Investigación en Genética Molecular, Centro Universitario de la Ciénega, Universidad de Guadalajara (CUCiénega-UdeG), Ocotlán, Jalisco, Mexico | |
| dc.description.abstract | Objectives: Some Single nucleotide polymorphisms (SNPs) of several candidate genes have been associated with low bone mineral density (BMD) and fracture risk. As the genetic variability of such SNPs in Hispanic and Native American populations is scarce, we analyzed the three SNPs that have been related with bone mass disorders (Sp1, A163G, and BsmI) located in the genes of Type I Collagen (COL1A1), Osteoprotegerin (OPG), and Vitamin D receptor (VDR) in Mexican Mestizos (people resulting from post-Columbian admixture) and five Amerindian populations. Methods: We genotyped these three SNPs by Polymerase chain reaction (PCR) and Restriction fragment length polymorphisms (RFLPs) in 523 individuals from five Mexican Amerindian groups (Nahua, Maya, Purépecha, Tarahumara, and Huichol) and 227 western Mestizos (Jalisco state). Results: The modal allele was the same in all the six populations for Sp1-COL1A1 (S > 77%), A163G-OPG (A > 80%), and BsmI-VDR (b > 62%). Genotype distribution was in Hardy-Weinberg equilibrium in all SNPs/populations, excepting Sp1-COL1A1 in the Purépecha group and BsmI-VDR in Mestizo. In terms of the presumably Sp1-COL1A1 risk allele to low BMD (allele "s"), the Purépecha group showed the highest allele (23%) and homozygous (14.5%) frequencies. If the role of this allele as a genetic predisposing factor to low BMD were confirmed, this would mean increased susceptibility of Purépechas with regard to Europeans (14.5 vs. 6.8%). Conclusions: This finding presumably could influence the genetic susceptibility to low BMD in Purépechas. For the SNPs, BsmI-VDR and A163G-OPG, relative homogeneity was observed among the Mexican populations analyzed here. © 2012 Wiley Periodicals, Inc. | |
