Sensitization of cervix cancer cells to Adriamycin by Pentoxifylline induces an increase in apoptosis and decrease senescence

dc.contributorAbeysekara, A.U., Department of Physics and Astronomy, Michigan State University, East Lansing, MI, United States; Alfaro, R., Instituto de Física, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Alvarez, C., CEFyMAP, Universidad Autónoma de Chiapas, Tuxtla Gutiérrez, Chiapas, Mexico; Álvarez, J.D., Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico; Arceo, R., CEFyMAP, Universidad Autónoma de Chiapas, Tuxtla Gutiérrez, Chiapas, Mexico; Arteaga-Velázquez, J.C., Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico; Ayala Solares, H.A., Department of Physics, Michigan Technological University, Houghton, MI, United States; Barber, A.S., Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, United States; Baughman, B.M., Department of Physics, University of Maryland, College Park, MD, United States; Bautista-Elivar, N., Universidad Politecnica de Pachuca, Pachuca, Hgo, Mexico; Belmont, E., Instituto de Física, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Benzvi, S.Y., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Berley, D., Department of Physics, University of Maryland, College Park, MD, United States; Bonilla Rosales, M., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; Braun, J., Department of Physics, University of Maryland, College Park, MD, United States; Caballero-Lopez, R.A., Instituto de Geofísica, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Carramiñana, A., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; Castillo, M., Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico; Cotti, U., Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico; Cotzomi, J., Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico; De La Fuente, E., Dept. de Fisica, IT.Phd (CUCEA), Universidad de Guadalajara, Jalisco, Mexico; De León, C., Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico; Deyoung, T., Department of Physics, Pennsylvania State University, University Park, PA, United States; Diaz Hernandez, R., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; Diaz-Velez, J.C., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Dingus, B.L., Physics Division, Los Alamos National Laboratory, Los Alamos, NM, United States; Duvernois, M.A., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Ellsworth, R.W., Department of Physics, University of Maryland, College Park, MD, United States, School of Physics, Astronomy and Computational Sciences, George Mason University, Fairfax, VA, United States; Fernandez, A., Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico; Fiorino, D.W., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Fraija, N., Instituto de Astronomía, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Galindo, A., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; García-Luna, J.L., Dept. de Fisica, IT.Phd (CUCEA), Universidad de Guadalajara, Jalisco, Mexico; García-Torales, G., Dept. de Fisica, IT.Phd (CUCEA), Universidad de Guadalajara, Jalisco, Mexico; Garfias, F., Instituto de Astronomía, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; González, L.X., Instituto de Geofísica, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; González, M.M., Instituto de Astronomía, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Goodman, J.A., Department of Physics, University of Maryland, College Park, MD, United States; Grabski, V., Instituto de Física, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Gussert, M., Colorado State University, Physics Dept., Ft Collins, CO 80523, United States; Hampel-Arias, Z., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Hui, C.M., Department of Physics, Michigan Technological University, Houghton, MI, United States; Hintemeyer, P., Department of Physics, Michigan Technological University, Houghton, MI, United States; Imran, A., Physics Division, Los Alamos National Laboratory, Los Alamos, NM, United States; Iriarte, A., Instituto de Astronomía, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Karn, P., Department of Physics and Astronomy, University of California, Irvine, Irvine, CA, United States; Kieda, D., Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, United States; Kunde, G.J., Physics Division, Los Alamos National Laboratory, Los Alamos, NM, United States; Lara, A., Instituto de Geofísica, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Lauer, R.J., Dept of Physics and Astronomy, University of New Mexico, Albuquerque, NM, United States; Lee, W.H., Instituto de Astronomía, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Lennarz, D., School of Physics and Center for Relativistic Astrophysics, Georgia Institute of Technology, Atlanta, GA 30332, United States; León Vargas, H., Instituto de Física, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Linares, E.C., Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico; Linnemann, J.T., Department of Physics and Astronomy, Michigan State University, East Lansing, MI, United States; Longo, M., Colorado State University, Physics Dept., Ft Collins, CO 80523, United States; Luna-García, R., Centro de Investigacion en Computacion, Instituto Politecnico Nacional, Mexico City, DF, Mexico; Marinelli, A., Instituto de Física, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Martinez, O., Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico; Martínez-Castro, J., Centro de Investigacion en Computacion, Instituto Politecnico Nacional, Mexico City, DF, Mexico; Matthews, J.A.J., Dept of Physics and Astronomy, University of New Mexico, Albuquerque, NM, United States; Miranda-Romagnoli, P., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico, Universidad Autónoma Del Estado de Hidalgo, Pachuca, Hidalgo, Mexico; Moreno, E., Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico; Mostafá, M., Colorado State University, Physics Dept., Ft Collins, CO 80523, United States; Nava, J., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; Nellen, L., Instituto de Ciencias Nucleares, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Newbold, M., Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, United States; Noriega-Papaqui, R., Universidad Autónoma Del Estado de Hidalgo, Pachuca, Hidalgo, Mexico; Oceguera-Becerra, T., Instituto de Física, Universidad Nacional Autónoma de México, Mexico D.F., Mexico, Dept. de Fisica, IT.Phd (CUCEA), Universidad de Guadalajara, Jalisco, Mexico; Patricelli, B., Instituto de Astronomía, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Pelayo, R., Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico; Pérez-Pérez, E.G., Universidad Politecnica de Pachuca, Pachuca, Hgo, Mexico; Pretz, J., Physics Division, Los Alamos National Laboratory, Los Alamos, NM, United States; Riviere, C., Instituto de Astronomía, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Ryan, J., Space Science Center, University of New Hampshire, Durham, NH, United States; Rosa-González, D., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; Salazar, H., Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla, Mexico; Salesa, F., Colorado State University, Physics Dept., Ft Collins, CO 80523, United States; Sandoval, A., Instituto de Física, Universidad Nacional Autónoma de México, Mexico D.F., Mexico; Santos, E., CEFyMAP, Universidad Autónoma de Chiapas, Tuxtla Gutiérrez, Chiapas, Mexico; Schneider, M., Santa Cruz Institute for Particle Physics, University of California, Santa Cruz, Santa Cruz, CA, United States; Silich, S., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; Sinnis, G., Physics Division, Los Alamos National Laboratory, Los Alamos, NM, United States; Smith, A.J., Department of Physics, University of Maryland, College Park, MD, United States; Sparks, K., Department of Physics, Pennsylvania State University, University Park, PA, United States; Springer, R.W., Department of Physics and Astronomy, University of Utah, Salt Lake City, UT, United States; Taboada, I., School of Physics and Center for Relativistic Astrophysics, Georgia Institute of Technology, Atlanta, GA 30332, United States; Toale, P.A., Department of Physics and Astronomy, University of Alabama, Tuscaloosa, AL, United States; Tollefson, K., Department of Physics and Astronomy, Michigan State University, East Lansing, MI, United States; Torres, I., Instituto Nacional de Astrofísica, óptica y Electrónica, Puebla, Mexico; Ukwatta, T.N., Department of Physics and Astronomy, Michigan State University, East Lansing, MI, United States; Villaseñor, L., Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Mexico; Weisgarber, T., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Westerhoff, S., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Wisher, I.G., Department of Physics, University of Wisconsin-Madison, Madison, WI, United States; Wood, J., Department of Physics, University of Maryland, College Park, MD, United States; Yodh, G.B., Department of Physics and Astronomy, University of California, Irvine, Irvine, CA, United States; Younk, P.W., Physics Division, Los Alamos National Laboratory, Los Alamos, NM, United States; Zaborov, D., Department of Physics, Pennsylvania State University, University Park, PA, United States; Zepeda, A., Physics Department, Centro de Investigacion y de Estudios Avanzados Del IPN, Mexico City, DF, Mexico; Zhou, H., Department of Physics, Michigan Technological University, Houghton, MI, United States
dc.creatorAbeysekara, A.U.
dc.creatorAlfaro, R.
dc.creatorAlvarez, C.
dc.creatorAlvarez, J.D.
dc.creatorArceo, R.
dc.creatorArteaga-Velazquez, J.C.
dc.creatorAyala Solares, H.A.
dc.creatorBarber, A.S.
dc.creatorBaughman, B.M.
dc.creatorBautista-Elivar, N.
dc.creatorBelmont, E.
dc.creatorBenzvi, S.Y.
dc.creatorBerley, D.
dc.creatorBonilla Rosales, M.
dc.creatorBraun, J.
dc.creatorCaballero-Lopez, R.A.
dc.creatorCarraminana, A.
dc.creatorCastillo, M.
dc.creatorCotti, U.
dc.creatorCotzomi, J.
dc.creatorDe La Fuente, E.
dc.creatorDe Leon, C.
dc.creatorDeyoung, T.
dc.creatorDiaz Hernandez, R.
dc.creatorDiaz-Velez, J.C.
dc.creatorDingus, B.L.
dc.creatorDuvernois, M.A.
dc.creatorEllsworth, R.W.
dc.creatorFernandez, A.
dc.creatorFiorino, D.W.
dc.creatorFraija, N.
dc.creatorGalindo, A.
dc.creatorGarcía-Luna, J.L.
dc.creatorGarcía-Torales, G.
dc.creatorGarfias, F.
dc.date.accessioned2015-09-15T18:54:59Z
dc.date.accessioned2022-11-02T15:21:12Z
dc.date.available2015-09-15T18:54:59Z
dc.date.available2022-11-02T15:21:12Z
dc.date.created2015-09-15T18:54:59Z
dc.date.issued2013
dc.identifierhttp://hdl.handle.net/20.500.12104/44466
dc.identifierhttp://www.scopus.com/inward/record.url?eid=2-s2.0-77952299096&partnerID=40&md5=ad6cefeeeecf0f99bfd227efef81f5a5
dc.identifier10.1186/1476-4598-9-114
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/5012769
dc.description.abstractThe High Altitude Water Cherenkov (HAWC) observatory is an array of large water Cherenkov detectors sensitive to gamma rays and hadronic cosmic rays in the energy band between 100 GeV and 100 TeV. The observatory will be used to measure high-energy protons and cosmic rays via detection of the energetic secondary particles reaching the ground when one of these particles interacts in the atmosphere above the detector. HAWC is under construction at a site 4100 meters above sea level on the northern slope of the volcano Sierra Negra, which is located in central Mexico at 19 N latitude. It is scheduled for completion in 2014. In this paper we estimate the sensitivity of the HAWC instrument to point-like and extended sources of gamma rays. The source fluxes are modeled using both unbroken power laws and power laws with exponential cutoffs. HAWC, in one year, is sensitive to point sources with integral power-law spectra as low as 5 10-13 cm-2sec-1 above 2 TeV (approximately 50 mCrab) over 5 sr of the sky. This is a conservative estimate based on simple event parameters and is expected to improve as the data analysis techniques are refined. We discuss known TeV sources and the scientific contributions that HAWC can make to our understanding of particle acceleration in these sources. " 2013 Elsevier B.V. All rights reserved.",,,,,,"10.1016/j.astropartphys.2013.08.002",,,"http://hdl.handle.net/20.500.12104/44464","http://www.scopus.com/inward/record.url?eid=2-s2.0-84887431030&partnerID=40&md5=b5d93bb6025b6d29331e2f6d3809a1ff",,,,,,,,"Astroparticle Physics",,"26
dc.description.abstract32",,"50-52",,"Scopus
dc.description.abstractWOS",,,,,,"Cosmic ray; TeV gamma-ray astronomy; Water cherenkov",,,,,,"Sensitivity of the high altitude water Cherenkov detector to sources of multi-TeV gamma rays",,"Article" "46236","123456789/35008",,"Jay, O., Centro de Estudios de Especies Menores, Universidad de Guantánamo, Carretera al Salvador km 6 1/2, Guantánamo, Cuba; Torres, V., Instituto de Ciencia Animal, Apartado Postal 24, San-José de las Lajas, Mayabeque, Cuba; Marrero, Y., Instituto de Ciencia Animal, Apartado Postal 24, San-José de las Lajas, Mayabeque, Cuba; Torres, J.P., Centro Universitario de Ciencias Biológicas y Agropecuarias, Universidad de Guadalajara, Las Agujas, Zapopan, Jalisco, Mexico",,"Jay, O.
dc.description.abstractTorres, V.
dc.description.abstractMarrero, Y.
dc.description.abstractTorres, J.P.",,"2012",,"In order to improve the ruminant feed evaluation techniques through a statistical method permitting the comparison of treatments in experiments of in vitro gas production, based on regression curves, data were collected from an experiment of in vitro gas production. The Gompertz function was fitted and the Monte Carlo simulation method was used, with the aim of analyzing the sensibility of four homogeneity tests of non-linear regression models. Thirteen new treatments were obtained, with 300 repetitions each and 280 points simulated per each repetition. The homogeneity tests were: extra sum of squares, Bayesian information criterion (BIC), Akaike information criterion (AIC), and Akaike information criterion corrected (AICC). The comparison criteria were assessed with modifications to the parameters, on the order of 1, 3, 5, and 10 %. The methodology for the comparison of the curves was based on the definition of the complete and the reduced models. The sensibility of each method was established according to the probability of fulfillment of the null hypothesis. The AICC showed higher sensibility, followed by the test of extra sum of squares. The AIC and the BIC were the least sensible. The parameter that affected the most the differences between treatments was the asymptotic coefficient (A).",,,,,,,,,"http://hdl.handle.net/20.500.12104/44457","http://www.scopus.com/inward/record.url?eid=2-s2.0-84879814468&partnerID=40&md5=361ce1a7ba1563364699cccfc8ffa32e",,,,,,"1",,"Cuban Journal of Agricultural Science",,"15
dc.description.abstract22",,"46",,"Scopus",,,,,,"Comparison of treatments; Extra sum of squares; Information criterion; Non-linear regression",,,,,,"Sensibility analysis of homogeneity tests of in vitro gas production curves by Monte Carlo simulation",,"Article" "46245","123456789/35008",,"Bravo-Cuellar, A., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico, Departamento Ciencias de la Salud, Centro Universitario de los Altos, Universidad de Guadalajara, Km 7, 5 Carretera a Yahualica, Tepatitlan de Morelos, Jalisco, CP 47820, Mexico; Ortiz-Lazareno, P.C., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico; Lerma-Diaz, J.M., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico; Dominguez-Rodriguez, J.R., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico; Jave-Suarez, L.F., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico; Aguilar-Lemarroy, A., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico; del Toro-Arreola, S., Laboratorio de Inmunología, Centro Universitario Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, puerta 7, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico; de Celis-Carrillo, R., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico; Sahagun-Flores, J.E., Laboratorio de Inmunología, Centro Universitario Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, puerta 7, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico, Hospital Regional Valentín Gómez Farías, Instituto de Seguridad Social y Servicios Sociales de los Trabajadores del Estado, Avenida Soledad Orozco 203, Colonia el Capullo, Guadalajara, Jalisco. CP 45150, Mexico; de Alba-García, J.E.G., Unidad de Investigación Social, Epidemiológica y Servicios de Salud, Centro Médico Nacional de Occidente, Instituto Mexicano del Seguro Social, Belisario Domínguez 1000, Colonia Independencia, Guadalajara, Jalisco, CP 44340, Mexico; Hernandez-Flores, G., División de Inmunología, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Sierra Mojada 800, Colonia Independencia. Guadalajara, Jalisco, CP 44340, Mexico",,"Bravo-Cuellar, A.
dc.description.abstractOrtiz-Lazareno, P.C.
dc.description.abstractLerma-Diaz, J.M.
dc.description.abstractDominguez-Rodriguez, J.R.
dc.description.abstractJave-Suarez, L.F.
dc.description.abstractAguilar-Lemarroy, A.
dc.description.abstractdel Toro-Arreola, S.
dc.description.abstractde Celis-Carrillo, R.
dc.description.abstractSahagun-Flores, J.E.
dc.description.abstractde Alba-García, J.E.G.
dc.description.abstractHernandez-Flores, G.",,"2010",,"Background: Chemotherapeutic drugs like Adriamycin (ADR) induces apoptosis or senescence in cancer cells but these cells often develop resistance and generate responses of short duration or complete failure. The methylxantine drug Pentoxifylline (PTX) used routinely in the clinics setting for circulatory diseases has been recently described to have antitumor properties. We evaluated whether pretreatment with PTX modifies apoptosis and senescence induced by ADR in cervix cancer cells.Methods: HeLa (HPV 18+), SiHa (HPV 16+) cervix cancer cells and non-tumorigenic immortalized HaCaT cells (control) were treated with PTX, ADR or PTX + ADR. The cellular toxicity of PTX and survival fraction were determinated by WST-1 and clonogenic assay respectively. Apoptosis, caspase activation and ADR efflux rate were measured by flow cytometry, senescence by microscopy. I?B? and DNA fragmentation were determinated by ELISA. Proapoptotic, antiapoptotic and senescence genes, as well as HPV-E6/E7 mRNA expression, were detected by time real RT-PCR. p53 protein levels were assayed by Western blot.Results: PTX is toxic (WST-1), affects survival (clonogenic assay) and induces apoptosis in cervix cancer cells. Additionally, the combination of this drug with ADR diminished the survival fraction and significantly increased apoptosis of HeLa and SiHa cervix cancer cells. Treatments were less effective in HaCaT cells. We found caspase participation in the induction of apoptosis by PTX, ADR or its combination. Surprisingly, in spite of the antitumor activity displayed by PTX, our results indicate that methylxantine, per se does not induce senescence; however it inhibits senescence induced by ADR and at the same time increases apoptosis. PTX elevates I?B? levels. Such sensitization is achieved through the up-regulation of proapoptotic factors such as caspase and bcl family gene expression. PTX and PTX + ADR also decrease E6 and E7 expression in SiHa cells, but not in HeLa cells. p53 was detected only in SiHa cells treated with ADR.Conclusion: PTX is a good inducer of apoptosis but does not induce senescence. Furthermore, PTX reduced the ADR-induced senescence and increased apoptosis in cervix cancer cells. " 2010 Bravo-Cuellar et al; licensee BioMed Central Ltd.
dc.relationScopus
dc.relationWOS
dc.relationMolecular Cancer
dc.relation9
dc.titleSensitization of cervix cancer cells to Adriamycin by Pentoxifylline induces an increase in apoptosis and decrease senescence
dc.typeArticle


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