Characterization of Cell Wall Extracts from Saccharomyces cerevisiae with Immunological Activity

Abstract

This work describes an electrochemical impedance spectroscopy (EIS) study of the hexane/CTAB-(CTA)4[Fe(CN)6]-butanol/water system in contact with a graphite electrode before and after being modified with an adsorbed surfactant layer in contact with an electroactive molecule. Six plausible equivalent circuits are tested in order to fit impedance experimental data for the unmodified electrode. Once an adsorbed surfactant layer is chemically attached to the graphite surface its electrochemical response changes. This type of modified graphite electrodes are tested in a Fe(CN) 6 3- standard solution using EIS. A series of three plausible equivalent circuits are casted to fit the experimental impedance data. The proposed models include diffusion of electroactive species through an adsorbed surfactant layer on the electrode surface. For all the equivalent circuits investigated a low ?2 fitting error and percentage of error determined from Kramers-Kronig plots are reported. Final proposals of an equivalent circuit to describe the unmodified and modified graphite electrode interface are presented. "The Electrochemical Society.",,,,,,"10.1149/1.3268401",,,"http://hdl.handle.net/20.500.12104/39986","http://www.scopus.com/inward/record.url?eid=2-s2.0-79959631427&partnerID=40&md5=28f753fdc36b7dd86e258f9f38fd0277",,,,,,"1",,"ECS Transactions",,"335

343",,"20",,"Scopus",,,,,,,,,,,,"Characterization for EIS of a modified graphite electrode in a hexane/CTAB-(CTA)4[Fe(CN)6]-butanol/water microemultion",,"Conference Paper" "41775","123456789/35008",,"Rodríguez F., J.G., Centro Universitario de Ciencias Biológicas Y Agropecuarias, Universidad de Guadalajara, Departamento de Producción agrícola, Las Agujas, Mpio. de Z., Km. 15.5, C. G.-Nogales, C.P. 45110, Jalisco, Mexico; Sánchez G., J.J., Centro Universitario de Ciencias Biológicas Y Agropecuarias, Universidad de Guadalajara, Departamento de Producción agrícola, Las Agujas, Mpio. de Z., Km. 15.5, C. G.-Nogales, C.P. 45110, Jalisco, Mexico; Baltazar M., B., Pioneer Hi-Bred International Inc., Camino Viejo a V. de Banderas, Km. 3, #19 Tapachula Nayarit, Mexico; De La Cruz L., L., Centro Universitario de Ciencias Biológicas Y Agropecuarias, Universidad de Guadalajara, Departamento de Producción agrícola, Las Agujas, Mpio. de Z., Km. 15.5, C. G.-Nogales, C.P. 45110, Jalisco, Mexico; Santacruz-Ruvalcaba, F., Centro Universitario de Ciencias Biológicas Y Agropecuarias, Universidad de Guadalajara, Departamento de Producción agrícola, Las Agujas, Mpio. de Z., Km. 15.5, C. G.-Nogales, C.P. 45110, Jalisco, Mexico; Ron P., J., Centro Universitario de Ciencias Biológicas Y Agropecuarias, Universidad de Guadalajara, Departamento de Producción agrícola, Las Agujas, Mpio. de Z., Km. 15.5, C. G.-Nogales, C.P. 45110, Jalisco, Mexico; Schoper, J.B., Pioneer Sementes Ltda., Unidade de Brasília, R. DF 250, KM 20, N. R. S. Dumont, Brasilia - DF, CEP: 73310-970, Brazil",,"Rodriguez, F., J.G.

Sanchez, G., J.J.

Baltazar, M., B.

De La Cruz, L., L.

Santacruz-Ruvalcaba, F.

Ron, P., J.

Schoper, J.B.",,"2006",,"Pollen and silk attributes as well as floral synchrony are the most important morphological features that influence pollination and gene flow in the genus Zea. To characterize floral morphology and synchrony, 115 teosinte populations, 60 maize accessions representing 53 landraces, three improved open pollinated varieties and 14 commercial hybrids were evaluated. A high level of variation among the Zea populations was found. The pollen size in landraces and commercial hybrids was larger (103-105 ?m) than in teosinte (84 ?m). Teosinte pollen grains of several accessions collapsed at pollen shed but for some populations viability was retained from 30 minutes to one hour. Silks in maize were twice as long as teosinte silks (16 to 27 cm vs 7.2-11.4 cm). Trichome density was five times higher in teosinte silks than in maize silks. Most indigenous teosinte (ssp. mexicana) populations from the Valley of Mexico and Central Plateau areas flowers synchronously with landraces and commercial hybrids. In contrast, tropical teosinte (ssp. parviglumis) flowers 2-3 weeks later than maize. The results of this study are important components to estimate the likelihood of gene flow among species of the genus Zea in Mexico.",,,,,,,,,"http://hdl.handle.net/20.500.12104/39996","http://www.scopus.com/inward/record.url?eid=2-s2.0-33746402419&partnerID=40&md5=33c1b97f8b92a516734e276bd92c4d9c",,,,,,"2",,"Maydica",,"383

398",,"51",,"Scopus

WOS",,,,,,"Gene flow; Genetic diversity; Maize; Pollen; Silks; Teosinte",,,,,,"Characterization of floral morphology and synchrony among Zea species in Mexico",,"Article" "41788","123456789/35008","Gallegos, A., Departamento de Ciencias Exactas y Tecnología, Centro Universitario de los Lagos, Universidad de Guadalajara, Enrique Díaz de León 1144, Colonia Paseos de la Montaña, 47460, Lagos de Moreno Jalisco, Mexico; Lucio, J.L., Departamento de Física, División de Ciencias e Ingenierías, Universidad de Guanajuato, Fraccionamiento Lomas del Campestre, Campus León, Lomas del Bosque 103, 37150, León Guanajuato, Mexico; Moreno, G., Departamento de Física, División de Ciencias e Ingenierías, Universidad de Guanajuato, Fraccionamiento Lomas del Campestre, Campus León, Lomas del Bosque 103, 37150, León Guanajuato, Mexico; Napsuciale, M., Departamento de Física, División de Ciencias e Ingenierías, Universidad de Guanajuato, Fraccionamiento Lomas del Campestre, Campus León, Lomas del Bosque 103, 37150, León Guanajuato, Mexico",,"Gallegos, A.

Lucio, J.L.

Moreno, G.

Napsuciale, M.",,"2010",,"We consider the forward-backward pion charge asymmetry for the e+e-??+?-e+e-??+?-? process. At tree level we consider bremsstrahlung and double resonance contributions. Although the latter contribution is formally sub-leading, it is enhanced at low dipion invariant mass due to ? resonant effects. We consider also four alternative models to describe the final state radiation at the loop level: Resonance Chiral Perturbation Theory, Unitarized Chiral Perturbation Theory, Kaon Loop Model and Linear Sigma Model. The last three models yield results compatible with experimental data. The Kaon Loop Model requires an energy dependent phase to achieve the agreement. © 2010 Elsevier B.V.",,,,,,"10.1016/j.physletb.2010.09.006",,,,"http://www.scopus.com/inward/record.url?eid=2-s2.0-77957009220&partnerID=40&md5=87eab78b46b467bea74e857e166929b7

http://hdl.handle.net/20.500.12104/40009",,,,,,"4",,"Physics Letters, Section B: Nuclear, Elementary Particle and High-Energy Physics",,"467

476",,"693",,"Scopus

WOS",,,,,,"Charge asymmetry; Chiral Lagrangians",,,,"Charge asymmetries in e+e-??+?-? at the ? resonance",,,,"Article", "41759","123456789/35008",,"Cooper-DeHoff, R.M., Univ. of Florida College of Medicine, Gainesville, FL, United States, University of Florida, Division of Cardiovascular Medicine, 1600 SW Archer Road, Gainesville, FL 32610-0277, United States; Handberg, E.M., Univ. of Florida College of Medicine, Gainesville, FL, United States; Cohen, J., St. Louis University, St. Louis, MO, United States; Kowey, P., Lankenau Hospital, Wynnewood, PA, United States; Messerli, F.H., Ochsner Clinic, New Orleans, LA, United States; Mancia, G., University Milano-Bicocca, Milano, Italy; Cangiano, J.L., Clinica Las Americas, Hato Rey, Puerto Rico; Gaxiola, E., Inst. Cardiovascular de Guadalajara, Guadalajara, Jalisco, Mexico; García-Barreto, D., Inst. Cardiol./Cirugia Cardiovasc., Havana, Cuba; Hewkin, A.C., Abbott Laboratories, Abbott Park, IL, United States; Pepine, C.J., Univ. of Florida College of Medicine, Gainesville, FL, United States",,"Cooper-DeHoff, R.M.

Handberg, E.M.

Cohen, J.

Kowey, P.

Messerli, F.H.

Mancia, G.

Cangiano, J.L.

Gaxiola, E.

García-Barreto, D.

Hewkin, A.C.

Pepine, C.J.",,"2004",,"Background: Despite a high prevalence of hypertension in the population with CAD, there are limited data describing the clinical characteristics and treatments, as well as their interrelations in these patients. This is particularly true for black and Hispanic patients who have been underrepresented in randomized CAD trials. Hypothesis: There exist racial and ethnic differences that define the characteristics of patients with both coronary artery disease (CAD) and hypertension. Methods: This report describes the characteristics of Caucasian, Hispanic, and black patients enrolled in the International Verapamil SR/trandolapril Study (INVEST), a prospective trial undertaken exclusively in patients with CAD and hypertension. Results: In all, 10,925 Caucasian, 8,045 Hispanic, and 3,029 black patients are described. An abnormal angiogram or documented myocardial infarction was observed more frequently in Caucasian patients (73%), while angina pectoris was more prevalent in Hispanic patients (87%). Diabetes and left ventricular hypertrophy were most common in black patients (33 and 29%, respectively), while hypercholesterolemia and prior revascularization (coronary artery bypass graft or angioplasty) were most common in Caucasian patients (64 and 41%, respectively). More than 60% of Hispanic and black patients were women-a unique characteristic for randomized CAD trials. Comparing race/ethnic cohorts, there were significant differences for all characteristics. More than 80% of patients in all race/ethnic groups were receiving antihypertensive therapy; however, only fewer than 25% had controlled blood pressure according to guidelines from the sixth report of the Joint National Committee on Prevention, Detection, Evaluation and Treatment of High Blood Pressure. Conclusions: This high-risk population of hypertensive patients with CAD has been undertreated and does not have well-controlled BP. Race/ethnic differences were observed for clinical characteristics and medication use.",,,,,,,,,"http://hdl.handle.net/20.500.12104/39980","http://www.scopus.com/inward/record.url?eid=2-s2.0-4644253100&partnerID=40&md5=f2f29857593512968dacd65db5d9edf6",,,,,,"10",,"Clinical Cardiology",,"571

576",,"27",,"Scopus",,,,,,"Black; Blood pressure control; Comorbidity; Coronary artery disease; Hispanic; Hypertension",,,,,,"Characteristics of contemporary patients with hypertension and coronary artery disease",,"Article" "44809","123456789/35008","Aguilar, J., Departamento de Ingeniería Química y de Química, Universidad de Guadalajara, Boul. M. García Barragán No. 1451, Guadalajara, Jal. 44430, Mexico; Rabelero, M., Departamento de Ingeniería Química y de Química, Universidad de Guadalajara, Boul. M. García Barragán No. 1451, Guadalajara, Jal. 44430, Mexico; Nuño-Donlucas, S.M., Departamento de Ingeniería Química y de Química, Universidad de Guadalajara, Boul. M. García Barragán No. 1451, Guadalajara, Jal. 44430, Mexico; Mendizábal, E., Departamento de Ingeniería Química y de Química, Universidad de Guadalajara, Boul. M. García Barragán No. 1451, Guadalajara, Jal. 44430, Mexico; Martínez-Richa, A., Departamento de Química, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, Gto. 36000, Mexico; López, R.G., Centro de Investigación en Química Aplicada, Boul. Ing. E. Reyna No. 140, Saltillo, Coah. 25253, Mexico; Arellano, M., Departamento de Ingeniería Química y de Química, Universidad de Guadalajara, Boul. M. García Barragán No. 1451, Guadalajara, Jal. 44430, Mexico; Puig, J.E., Departamento de Ingeniería Química y de Química, Universidad de Guadalajara, Boul. M. García Barragán No. 1451, Guadalajara, Jal. 44430, Mexico

Mendizábal, E. M., Universidad de Guadalajara. Centro Universitario de Ciencias Exactas e Ingenierías",,"Aguilar, J.

Rabelero, M.

Nuno-Donlucas, S.M.

Mendizábal, E. M.

Martinez-Richa, A.

Lopez, R.G.

Arellano, M.

Puig, J.E.",,"2011",,"The effect of surfactant (sodium dodecyl sulfate) concentration on particle size, molar masses, glass transition, and tacticity of poly(methyl methacrylate) (PMMA) nanoparticles synthesized by semicontinuous heterophase polymerization under monomer-starved condition at constant monomer feeding rate is reported. Starved conditions are confirmed by the low amount of residual monomer throughout the reaction and by the fact that the instantaneous polymerization rate is similar to the feeding rate of monomer. Under these conditions, polymer particles in the nanometer range (20-30 nm) were obtained with narrow size distribution (1.07 < Dw/Dn < 1.18), depending of surfactant concentration. Final particle size diminishes as the surfactant concentration is increased. Glass transition temperatures and syndiotactic content (54%-59%) of the produced polymers are substantially higher than those reported for commercial and bulk-made PMMA. Molar masses are much lower than those expected from termination by chain transfer to monomer, which is the typical termination mechanism in 0-1 emulsion and microemulsion polymerization of this monomer. © 2010 Wiley Periodicals, Inc.",,,,,,"10.1002/app.32886",,,,"http://www.scopus.com/inward/record.url?eid=2-s2.0-78249235323&partnerID=40&md5=a78afc3e585bfcf6925418f193d1c48e

http://hdl.handle.net/20.500.12104/43030",,,,,,"3",,"Journal of Applied Polymer Science",,"1827

1834",,"119",,"Scopus

WOS",,,,,,"nanotechnology; particle size distribution; radical polymerization; syndiotactic",,,,"Narrow size-distribution poly(methyl methacrylate) nanoparticles made by semicontinuous heterophase polymerization",,,,"Article", "41786","123456789/35008",,"David, D., Department of Genetics, National Institute of Health Dr Ricardo Jorge, Lisboa, Portugal; Marques, B., Department of Genetics, National Institute of Health Dr Ricardo Jorge, Lisboa, Portugal; Ferreira, C., Department of Genetics, National Institute of Health Dr Ricardo Jorge, Lisboa, Portugal; Vieira, P., Department of Genetics, National Institute of Health Dr Ricardo Jorge, Lisboa, Portugal; Corona-Rivera, A., Department of Molecular Biology and Genomics, University of Guadalajara, Guadalajara, Mexico; Ferreira, J.C., Obstetrics Department, Prenatal Diagnosis Centre, García de Orta Hospital, Almada, Portugal; van Bokhoven, H., Department of Human Genetics, Nijmegen Centre for Molecular Life Sciences, Radboud University, Nijmegen, Netherlands",,"David, D.

Marques, B.

Ferreira, C.

Vieira, P.

Corona-Rivera, A.

Ferreira, J.C.

van Bokhoven, H.",,"2009",,"Split hand-split foot malformation or ectrodactyly is a heterogeneous congenital defect of digit formation. The aim of this study is the mapping of the breakpoints and a detailed molecular characterization of the candidate genes for an isolated and syndromic form of ectrodactyly, both associated with de novo apparently balanced chromosome translocations involving the same chromosome 2 band, [t(2;11)(q14.2;q14.2)] and [t(2;4)(q14.1;q35)], respectively. Breakpoints were mapped by fluorescence in situ hybridization using bacterial artificial chromosome clones. Where possible, these breakpoints were further delimited. Candidate genes were screened for pathogenic mutations and the expression levels of two of them analysed. The isolated bilateral split foot malformation-associated chromosome 2 breakpoint was localized at 120.9Mb, between the two main candidate genes, encoding GLI-Kruppel family member GLI2 and inhibin-?B. The second breakpoint associated with holoprosencephaly, hypertelorism and ectrodactyly syndrome was mapped 2.5Mb proximal at 118.4Mb and the candidate genes identified from this region were the insulin-induced protein 2 and the homeobox protein engrailed-1. No clear pathogenic mutations were identified in any of these genes. The breakpoint between INHBB and GLI2 coincides with a previously identified translocation breakpoint associated with ectrodactyly. We propose a mechanism by which translocations in the 2q14.1-q14.2 region disrupt the specific arrangement of long-range regulatory elements that control the tight quantitative spatiotemporal expression of one or more genes from the breakpoint region.",,,,,,"10.1038/ejhg.2009.2",,,"http://hdl.handle.net/20.500.12104/40007","http://www.scopus.com/inward/record.url?eid=2-s2.0-67749094763&partnerID=40&md5=132c187207c771fa5d5db20a6ceaf0bd",,,,,,"8",,"European Journal of Human Genetics",,"1024

1033",,"17",,"Scopus

WOS",,,,,,,,,,,,"Characterization of two ectrodactyly-associated translocation breakpoints separated by 2.5Mb on chromosome 2q14.1-q14.2",,"Article" "41768","123456789/35008",,"Aguilar, B., Laboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara (UdG), Boulevard Marcelino García Barragán 1421, 44420 Guadalajara, Jalisco, Mexico; Solís, J., Laboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara (UdG), Boulevard Marcelino García Barragán 1421, 44420 Guadalajara, Jalisco, Mexico; Viveros, J.M., Laboratorio de Microbiología Industrial, Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara (UdG), Boulevard Marcelino García Barragán 1421, 44420 Guadalajara, Jalisco, Mexico; López, Z., Cell Biology Laboratory, Centro Universitario de la Ciénega, UdG, Ocotlán, Jalisco, Mexico; Knauth, P., Cell Biology Laboratory, Centro Universitario de la Ciénega, UdG, Ocotlán, Jalisco, Mexico",,"Aguilar, B.

Solis, J.

Viveros, J.M.

Lopez, Z.

Knauth, P.",,"2012",,"The yeast cell wall (YCW) is composed mainly of ?-glucans, mannoproteins, and chitin. ?-Glucans are well known immunomodulators, recognized by Toll-like receptor-4 (TLR-4) and inducing the nuclear factor-kappaB (NF-?B) signaling pathway, which is implicated in the synthesis of pro-inflammatory mediators such as interleukin-1beta (IL-1?), tumor necrosis factor-alpha (TNF-?), and nitric oxide (NO). Here we evaluated the immunomodulating effect of the YCW extracts from three different strains of Saccharomyces cerevisiae (AR5, MG, and L013) obtained from Tequila and bread-making processes by challenging intraperitoneal macrophages from BALB/c mice with YCW extract and/or lipopolysaccharide (LPS) endotoxin from Escherichia coli. Only the extract from AR5, which had a high glucan-to-mannan ratio, exhibited an antagonistic effect upon LPS stimulation: The IL-1? and TNF-? concentrations in plasma increased slightly by 24% and 4%, respectively, and the NO level increased moderately by 200% compared with the control. When the YCW extracts had less glucan, as found for MG and L013, the LPS stimulus had more of a synergistic effect on the plasma IL-1? concentration, which increased about 175%, and on the NO level, which rose 330-470%. These results indicated that under certain conditions, YCW extracts have an immune-attenuating effect; that is, the mice liberated much less IL-1? or nearly equal amounts of TNF-? or NO compared with the LPS stimulus alone. " 2012 Copyright Taylor & Francis Group, LLC.