| dc.contributor | Ortuño-Sahagún, D., Laboratorio de Desarrollo y Regeneración Neural, Instituto de Neurobiología, C.U.C.B.A, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Rivera-Cervantes, M.C., Departamento de Biología Celular y Molecular, Laboratorio de Neurobiología Celular, C.U.C.B.A, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Gudiño-Cabrera, G., Laboratorio de Desarrollo y Regeneración Neural, Instituto de Neurobiología, C.U.C.B.A, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico; Junyent, F., Unitat de Farmacologia i Farmacognòsia Facultat de Farmàcia, Institut de Biomedicina (IBUB), Universitat de Barcelona, Centros de Investigacion Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain; Verdaguer, E., Unitat de Farmacologia i Farmacognòsia Facultat de Farmàcia, Institut de Biomedicina (IBUB), Universitat de Barcelona, Centros de Investigacion Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain; Auladell, C., Departament de Biologia Celular, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain; Pallàs, M., Unitat de Farmacologia i Farmacognòsia Facultat de Farmàcia, Institut de Biomedicina (IBUB), Universitat de Barcelona, Centros de Investigacion Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain; Camins, A., Unitat de Farmacologia i Farmacognòsia Facultat de Farmàcia, Institut de Biomedicina (IBUB), Universitat de Barcelona, Centros de Investigacion Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain; Beas-Zárate, C., Laboratorio de Desarrollo y Regeneración Neural, Instituto de Neurobiología, C.U.C.B.A, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico, Laboratorio de Neurobiología Celular y Molecular, División de Neurociencias, CIBO, IMSS, Guadalajara, Jalisco, Mexico | |
| dc.description.abstract | Multiple factors are involved in the glutamate-induced excitotoxicity phenomenon, such as overload of ionotropic and metabotropic receptors, excess Ca 2+ influx, nitric oxide synthase activation, oxidative damage due to increase in free radicals, and release of endogenous polyamine, among others. In order to attempt a more integrated approach to address this issue, we established, by microarray analysis, the hippocampus gene expression profiles under glutamate-induced excitotoxicity conditions. Increased gene expression is mainly related to excitotoxicity (CaMKII, glypican 2, GFAP, NCX3, IL-2, and Gmeb2) or with cell damage response (dynactin and Ecel1). Several genes that augmented their expression are related to glutamatergic system modulation, in particular with NMDA receptor modulation and calcium homeostasis (IL-2, CaMKII, acrosin, Gmeb2, hAChE, Slc83a, and SP1 factor). Conversely, among genes that diminished their expression, we found the Syngap 1, which is downregulated by CaMKII, and the MHC II, which is downregulated by glutamate. Changes observed in gene expression induced by monosodium glutamate (MSG) neonatal treatment in the hippocampus are consistent with the activation of the mechanisms that modulate NMDA receptor function as well as with the implementation of plastic response to cell damage and intracellular calcium homeostasis. Regarding this aspect, we report here that NCX3/Slc8a3, a Na +/Ca 2+ membrane exchanger, is highly expressed in astrocytes, both in vitro and in vivo, in response to glutamate-induced excitotoxicity. Hence, the results of this analysis present a broad view of the expression profile elicited by MSG neonatal treatment, and lead us to suggest the possible molecular pathways of action and reaction involved under this experimental model of excitotoxicity. © 2010 Wiley Periodicals, Inc. | |
