Technetium 99m-labeled annexin V scintigraphy of platelet activation in vegetations of experimental endocarditis

Abstract

The effect of pressure and temperature on the synthesis of cobalt-molybdenum oxides and their characterisation is herein reported. The synthesis was performed under hydrothermal conditions without any template. It was found that the experimental conditions affect the physical properties of the CoMoO4. SEM images demonstrate that the synthesised powder is comprised of rods, and the width of the structures is about 100 nm but the length varies from about 5 ?m to less than 1 ?m. Low temperature and pressure favour the formation of longer rods. " 2013 Elsevier Ltd. All rights reserved.",,,,,,"10.1016/j.materresbull.2013.07.007",,,"http://hdl.handle.net/20.500.12104/44986","http://www.scopus.com/inward/record.url?eid=2-s2.0-84883328859&partnerID=40&md5=9a9dbfd86ec46a724d137f03d4e1fbfe",,,,,,"11",,"Materials Research Bulletin",,"4614

4617",,"48",,"Scopus

WOS",,,,,,"A. Metals; A. Oxides; B. Chemical synthesis; C. X-ray diffraction",,,,,,"Template-free synthesis of CoMoO4 rods and their characterization",,"Article" "46742","123456789/35008",,"Dávila, R., Department of Computer Science and Engineering, CUValles, Universidad de Guadalajara, Mexico; Hernández, S.C., Department of Information Systems, CUCEA, Universidad de Guadalajara, Mexico; Corona, J.F., Department of Mechanical and Industrial Engineering, ITESM, Campus Guadalajara, Mexico",,"Davila, R.

Hernandez, S.C.

Corona, J.F.",,"2007",,"Teaching engineering students courses such as computer science theory, automata theory and discrete mathematics took us to realize that introducing basic notions of logic, especially following Gentzen's natural deduction approach [6], to students in their first year, brought a benefit in the ability of those students to recover the structure of the arguments presented in text documents, cartoons and newspaper articles. In this document we propose that teaching logic help the students to better justify their arguments, enhance their reasoning, to better express their ideas and will help them in general to be more consistent in their presentations and proposals.",,,,,,,,,"http://hdl.handle.net/20.500.12104/44963","http://www.scopus.com/inward/record.url?eid=2-s2.0-84871557502&partnerID=40&md5=04a520acccc22d72d32018f6c7c79583",,,,,,,,"CEUR Workshop Proceedings",,,,"286",,"Scopus",,,,,,"Deductive reasoning; Education; Learning; Logic; Natural deduction",,,,,,"Teaching natural deduction to improve text argumentation analysis in engineering students",,"Conference Paper" "46750","123456789/35008",,,,"Vogt, W.",,"1990",,,,,,,,,,"0185-3929","http://hdl.handle.net/20.500.12104/44971",,,"Español",,,,"42",,"Estudios de historia y sociedad",,"101-111",,"11",,"CLASE",,,,,,,,"Historia de la literatura",,,,"Influencias extranjeras en la literatura mexicana anterior a la revolución de 1910",,"journalArticle" "46746","123456789/35008",,"Rouzet, F., AP-HP, Groupe Hospitalier Bichat-Claude Bernard, UMR S773, INSERM U773, Paris, France, Service de Medecine Nucleaire, Hospital Bichat, 46 rue Henri-Huchard, 75018 Paris, France; Hernandez, M.D., INSERM U698, Cardiovascular Remodeling, Paris 7 University, Paris, France, Department of Pathology, University of Guadalajara, Guadalajara, Mexico; Hervatin, F., CEA-DRM-SHFJ, Orsay, France; Sarda-Mantel, L., AP-HP, Groupe Hospitalier Bichat-Claude Bernard, UMR S773, INSERM U773, Paris, France; Lefort, A., EA3964, University Denis Diderot-Paris 7, Paris, France; Duval, X., INSERM CIC 007, AP-HP, University Denis Diderot-Paris 7, Paris, France; Louedec, L., INSERM U698, Cardiovascular Remodeling, Paris 7 University, Paris, France; Fantin, B., EA3964, University Denis Diderot-Paris 7, Paris, France; Le Guludec, D., AP-HP, Groupe Hospitalier Bichat-Claude Bernard, UMR S773, INSERM U773, Paris, France; Michel, J.-B., INSERM U698, Cardiovascular Remodeling, Paris 7 University, Paris, France",,"Rouzet, F.

Hernandez, M.D.

Hervatin, F.

Sarda-Mantel, L.

Lefort, A.

Duval, X.

Louedec, L.

Fantin, B.

Le Guludec, D.

Michel, J.-B.",,"2008",,"BACKGROUND - The pathophysiology of infective endocarditis involves a pathogen/host tissue interaction, leading to formation of infected thrombotic vegetations. Annexin V is a ligand of phosphatidylserines exposed by activated platelets and apoptotic cells. Because vegetations are platelet-fibrin clots in which platelet proaggregant activity is enhanced by bacterial colonization, we investigated the ability of annexin V labeled with technetium Tc 99m (Tc-ANX) to provide functional imaging of these vegetations in experimental models of infective endocarditis. This ability was assessed in rabbits and rats because of the different interest of these 2 species in preclinical analysis. METHODS AND RESULTS - Nonbacterial thrombotic endocarditis was induced with the use of a catheter left indwelling through the aortic or tricuspid valve, and animals were injected with either a bacterial inoculum or saline. Scintigraphic investigations were performed 5 days later and showed a higher Tc-ANX uptake by vegetations in infected versus noninfected animals (ratio, 1.3 for in vivo acquisitions and 2 for autoradiography; P<0.0001 for all), whereas no significant uptake was present in controls. Right-sided endocarditis was associated with pulmonary uptake foci corresponding to emboli. Histological analysis of vegetations showed a specific uptake of Tc-ANX at the interface between circulating blood and vegetation. In parallel, underlying myocardial tissue showed myocyte apoptosis and mucoid degeneration, without extracellular matrix degradation at this stage. CONCLUSIONS - Tc-ANX is suitable for functional imaging of platelet-fibrin vegetations in endocarditis, as well as embolic events. Tc-ANX uptake reflects mainly platelet activation in the luminal layer of vegetations. This uptake is enhanced by bacterial colonization. " 2008 American Heart Association, Inc.