Efecto de la testosterona sobre el desarrollo de las espículas peneanas en cobayos y su influencia en la fertilidad

Abstract

The present investigation determined the relationship between the levels of total testosterone (TT) and the development of the penile spicules (EPs); in addition, evaluated the effect of the application of exogenous testosterone on the regeneration of Eps; and finally, evaluated the influence of EPs on the fertility and prolificacy of guinea pigs. Three experiments were divided into groups: Experiment 1 (Group 1 integers, n = 25; Group 2 extirpated EPs, n = 25; Group 3 castrated, n = 25); Experiment 2 (Group 1 castrated + 250ug of Testosterone Enanthate, n = 15; Group 2 castrated + 125ug of Testosterone Enanthate, n = 15; Group 3 integers, n = 15); Experiment 3 (Group 1 integers + females, n = 5 + 25, Group 2 extirpated EPs + females, n = 5 + 25). The TT was determined by RIA, the length of the EPs by means of an Excelis camera AU-600-HD mounted to a microscope (100x) by the software AmScope V.3.7 and the fertility and prolificacy was evaluated by the number of pregnant females and the number of babies born. Determining that the length of the EP depends on the TT levels; however, if EPs are excised in guinea pigs with functional testes, the levels of TT generated by the gonads are not sufficient to produce regeneration of the EPs; whereas, if EPs suffer a regression process due to castration, they can be regenerated with exogenous testosterone; On the other hand, EPs do not influence the percentage of fertility, but they do have a significant influence on the percentage of prolificacy, which is not related to low levels of TT in animals that are extirpated.