ARTÍCULO DE CONFERENCIA
Effect of L-carnitine in a skimmed milk based-diluent on membranes preservation and kinetic activity of ram sperm under chilled conditions
Fecha
2019Autor
Galarza Lucero, Diego Andres
Lopez Sebastian, Antonio
Santiago Moreno, Julián
Institución
Resumen
The synergic effect of L-carnitine (LC) and skimmed milk base-extender (UHT) on the kinetic parameters and membranes integrity of cold-stored ram sperm was evaluated. For this purpose, twelve pools of thirty-six semen ejaculates that were collected with artificial vagina from twelve Merino rams were used. After initial evaluation, the semen was divided into 6 aliquots enough to diluted with UHT-extender (control) and 1mM (UHT-LC1), 2.5mM (UHT-LC2.5), 5mM (UHT-LC5), 7,5mM (UHT-LC7.5) and 10mM (UHT-LC10) of LC, respectively, at 200 x 106 cells/ml concentration. Semen samples were cold-stored (5ºC) up to 96 h. Kinetic variables and membranes integrity were assessed by CASA system (SCA) and triple fluorescence association test (IP/PNA-FITC/Mitotracker green). An ANOVA-one way and Bonferroni´s test were used to evaluated effects of LC doses. Overall, the total motility (TM, %), straight line velocity (VSL, μm/s) and total sperm with plasma and acrosome membranes integrity and high mitochondrial function (IPIAHM, %) were greater with all doses of LC (1 to 10 mM) than control group. Also, the UHT-LC5 group provided better values than others LC groups according to motilities. In conclusion, LC improves kinetic vigor and provides an antioxidant protective effect to sperm membranes of cold-stored ram when added to skimmed milk based-diluent