Identificación y genotipificación de los tipos de virus de papiloma bovino en sangre periférica u orina de animales afectados por Hematuria vesical enzoótica bovina

Abstract

In the development of malignant neoplasms in the urinary bladder in cattle, two factors are combined, on the one hand, chronic intoxication with Ptaquiloside present in ferns of the genus Pteridium and infection with bovine papillomavirus (BoPV). The presence of the infection can be determined using molecular biology tools in post-mortem collected samples. Therefore, the aim of this study was to identify the presence of BoPV in samples of urinary bladder, blood and urine tissues to evaluate their use as clinical samples for ante-mortem diagnostic; for which samples of bovine papillomatosis associated with bovine enzootic bladder hematuria (HVEB) were analyzed, collecting 132 samples of urine, urinary bladder tissue and blood of 53 animals, sampled in the municipal camals of Azogues, Cuenca and Paute, using PCR and RFLP as diagnostic tests. To Válidate the BoPV detection test, a process control was performed for each of the mentioned samples, which allowed the identification of 126 valid samples. It was only possible to identify the presence of a mixture of BoPV type 3 and 6 in one of the collected blood samples, whereas in the samples of urine and bladder tissue its presence could not be determined; The BoPVs identified agree with the types of BoPV already described in blood. Due to the limited results of the present study, it is necessary to deepen the study, orienting it towards the identification of genetic material of BoPV in samples of neoplastic lesions present in the urinary bladder of old animals.