bachelorThesis
Determinación de la actividad antibacteriana de Euglena viridis y oscillatoria sp sobre staphylococcus y escherichia coli
Fecha
2014Autor
Campoverde Alvarado, María Verónica
Pomaquiza Lema, Gloria verónica
Institución
Resumen
This study was conducted in order to determine the antibacterial activity of chloroform and methanol extracts of the microalga Euglena viridis and of the cyanobacteriumOscillatoriasp against bacterial strains of Escherichia coli and Staphylococcus aureus, two bacterial species causing of multiples diseases in humans.
To the isolation and cultivation of microalgae, the WC and BG-11 solid and liquid media was used, specifics to the development of freshwater microalgae and cyanobacteria respectively, these were allowed under controlled lighting, temperature and agitation to facilitate their growth and development.
The extracts to this study were obtained by ultrasound-assisted maceration, to be concentratedunder vacuum in the case of the chloroform extracts, and the methanol extracts by lyophilization of both algae.
To microbiological analysis, Agar diffusion well-variant was used, which is semi-quantitative and allows determining the minimum inhibitory concentration (MIC) of compounds of plant origin, by measuring the zones of inhibition around each well.
The results indicate the methanolic and chloroform extracts of Euglena viridis at concentrations of 6 and 4 mg/mL respectively do not have antibacterial activity against Escherichia coli and Staphylococcus aureus, similarly to Oscillatoriasp in which the antibacterial activity was tested in concentrations of 4 and 2 mg/mL for methanolic and chloroform extracts respectively.