Article
Cultivo in vitro de Scoparia dulcis L. (Scrophulariaceae)
Fecha
2009-06Autor
Cerna, Marco
Tafur, Valdano
Institución
Resumen
In the framework of this investigation the techniques were designed for the propagation of meristematic tissue
present in the bud of the nodal segments of the species, Scoparia dulcis L. (Scrophulariaceae), thereby
obtaining plants genetically identical to the mother plant. They were identical to the plant mother.These techniques
help to reduce the space and time necessary for the cultivation this species. The protocol thus developed
will produce specimens of high performance in active principles and free of infectious agents, particularly
for the production of material required for medicinal products. Scoparia dulcis L. has been described with
anti-malarial proprieties. One of its chemical components is beta sitosterol, which is used to cure prostate
inflammation and constitutes a base element for the treatment of baldness. The research was developed in
several stages: first, the establishment of a protocol for bacterial and mushroom disinfection of explants. With
this purpose the times of explants exposition to sodium hypochlorite 2,25% and 3% were tested. The second
stage was to determine the ideal in vitro cultivation environment. The basic environment was Murashige and
Skoog, to which several growth hormones (IBA, ANA y 2,4-D) were added. The last stage was to test the adaptation
of the species to a rustic low-level control environment. Explant disinfection was conducted in sodium
hypochlorite 3% for 23 minutes. in vitro cultivation required a basic environment of Murashige and Skoog without
growth hormones.