bachelorThesis
Grado de la mastitis bovina y su correlación con el conteo de células somáticas diferenciadas de unidades productivas de la sierra norte de la provincia de Pichincha
Fecha
2022-07Autor
López Constante, Andrea Elizabeth
Mendoza Quinzo, Erika Lisseth
Institución
Resumen
Bovine mastitis presents as inflammation of the mammary gland. It is
characterized by the presence of redness, heat, and pain, clearly visible signs in the udder
of the animal. In turn, it develops the partial loss of function of the quarters of the
mammary gland. For the diagnosis of breast cancer, it can be divided into clinical and
subclinical groups. The indirect field test, the California Mastitis Test (CMT), was used
together with somatic cell count (CCS) being the number of cells present in milk
commonly expressed in milliliters (cells/mL) to measure the disease. The differential
somatic cell count (DSCC) consists of lymphocytes, nuclear polymorph and
macrophages, which allow for the detection and control of mastitis. For this study, 499
samples were obtained from four collection centers in Cayambe and livestock companies
in the province of Pichincha. The CMT test was performed for subsequent analysis in the
FOSSOMATIC 7DC by flow cytometry at the UPS Milk Quality Laboratory with CCS
and DSCC parameters, making it possible to determine the degree of bovine mastitis in
which the dairy herd is present. In the relationship between CMT and CCS, the highest
percentage of cases was negative in a range of 0 – 200 000 cells/mL indicating that they
are healthy udders, only one case > 5 000 000 cells/mL was reported as chronic mastitis
in small and medium producers. The correlation of CCS with DSCC and the degree of
bovine mastitis showed a high number of positive cases for subclinical or acute mastitis
(Quadrant C). The ANOVA statistical analysis revealed a low relationship between
DSCC and CCS, as differential somatic cells do not increase linearly.