bachelorThesis
Evaluación de la respuesta fisiológica de la microalga Chlorella sp. a la presencia de metal cromo (VI) en aguas sintéticas.
Fecha
2019-06Autor
Mena Bolaños, Daniela Victoria
Institución
Resumen
Heavy metal as chromium are among the most toxic contaminants that affect the water
resources due of the textile and tannery activities involve damage in the microalgae
that shape the first links of the trophic chain. The present work evaluates the
physiological response of the Chlorella sp. in the presence of chromium (VI) in
synthetic waters. For this was quantified chlorophyll a, b and carotenoids for
spectrophometers methods and the mortality percent through counts in Neubauer
chamber, the microalgae was exposed to five concentrations of Cr (VI) in a period of
96 hours at pH 4, 6 and 8. The inhabitation synthesis of pigments and cellular death
were determined for the concentrations higher than 15 mg/L of chromium meanwhile
lower concentrations increase the cell density and pigments. The pH 4 affects
Chlorella sp. with a mortality percent of 67 % whit a metal concentration to 60 mg/L
while pH 6 and 8 had a mortality percent to 27 % and 17% respectively. The facts of
pH and metal concentration have been fundamental since in an a extrapolation with
10x106 cell/mL it was observed that the physiology of microalgae was affected in pH
4 and 6 with less production of pigments and pH 8 was optimum for that Chlorella sp.
can resist high concentrations of metal.