dc.date.accessioned | 2021-08-23T22:53:37Z | |
dc.date.accessioned | 2022-10-19T00:21:42Z | |
dc.date.available | 2021-08-23T22:53:37Z | |
dc.date.available | 2022-10-19T00:21:42Z | |
dc.date.created | 2021-08-23T22:53:37Z | |
dc.date.issued | 2018 | |
dc.identifier | http://hdl.handle.net/10533/251218 | |
dc.identifier | 1151025 | |
dc.identifier | WOS:000443539100016 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4482481 | |
dc.description.abstract | Background: The spore is the virulence factor identified to be involved in the recurrence of the disease caused by Clostridium difficile. Objectives: To demonstrate that lethal antibiotic concentrations induce the appearance of C. difficile persister-like non-spore cells. Methods: C. difficile and derivative spo0A mutant strains were tested for their susceptibility to antibiotics, as determined using an agar dilution method. Persister-cell generation was determined for all strains using up to 10x the MIC of every antibiotic for up to 6 days. Results: Using up to 10x the MIC of every antibiotic, we were able to induce the appearance of persister-like behaviour since biphasic killing curves could be observed in response to treatment antibiotics. Conclusions: To the best of our knowledge, this work provides, for the first time, experimental evidence of the appearance of C. difficile persister-like cells, opening a new research avenue in the pathogenesis of this nosocomial pathogen. | |
dc.language | eng | |
dc.relation | https://doi.org/10.1093/jac/dky186 | |
dc.relation | handle/10533/111557 | |
dc.relation | 10.1093/jac/dky186 | |
dc.relation | handle/10533/111541 | |
dc.relation | handle/10533/108045 | |
dc.rights | info:eu-repo/semantics/article | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.rights | Atribución-NoComercial-SinDerivadas 3.0 Chile | |
dc.rights | http://creativecommons.org/licenses/by-nc-nd/3.0/cl/ | |
dc.title | Effect of antibiotic treatment on the formation of non-spore Clostridium difficile persister-like cells | |
dc.type | Articulo | |