dc.creatorGregorio, Cristian de [Ctr Integrat Biol, Fac Sci, Universidad Mayor, Chile]
dc.creatorDíaz, Paula [Ctr Integrat Biol, Fac Sci, Universidad Mayor, Chile]
dc.creatorLópez-Leal, Rodrigo [Ctr Integrat Biol, Fac Sci, Universidad Mayor, Chile]
dc.creatorManque, Patricio [Ctr Integrat Biol, Fac Sci, Universidad Mayor, Chile]
dc.creatorCourt, Felipe A. [Ctr Integrat Biol, Fac Sci, Universidad Mayor, Chile]
dc.date.accessioned2021-02-03T19:10:43Z
dc.date.accessioned2022-10-18T18:42:55Z
dc.date.available2021-02-03T19:10:43Z
dc.date.available2022-10-18T18:42:55Z
dc.date.created2021-02-03T19:10:43Z
dc.date.issued2018
dc.identifierDe Gregorio, C., Díaz, P., López-Leal, R., Manque, P., & Court, F. A. (2018). Purification of Exosomes from Primary Schwann Cells, RNA Extraction, and Next-Generation Sequencing of Exosomal RNAs. Methods in molecular biology (Clifton, N.J.), 1739, 299–315. https://doi.org/10.1007/978-1-4939-7649-2_19
dc.identifier1940-6029
dc.identifiereISSN: 1064-3745
dc.identifierhttp://repositorio.umayor.cl/xmlui/handle/sibum/7336
dc.identifierhttps://doi.org/10.1007/978-1-4939-7649-2_19
dc.identifierhttps://pubmed.ncbi.nlm.nih.gov/29546715/
dc.identifierhttps://link.springer.com/protocol/10.1007%2F978-1-4939-7649-2_19#citeas
dc.identifier10.1007/978-1-4939-7649-2_19
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4455120
dc.description.abstractExosomes are small (30-150 nm) vesicles of endosomal origin secreted by most cell types. Exosomes contain proteins, lipids, and RNA species including microRNA, mRNA, rRNA, and long noncoding RNAs. The mechanisms associated with exosome synthesis and cargo loading are still poorly understood. A role for exosomes in intercellular communication has been reported in physiological and pathological conditions both in vitro and in vivo. Previous studies have suggested that Schwann cell-derived exosomes regulate neuronal functions, but the mechanisms are still unclear. Here, we describe protocols to establish rat neonatal Schwann cell cultures and to isolate exosomes from the conditioned medium of these cultures by differential ultracentrifugation. To analyze the RNA content of Schwann cell-derived exosomes, we detail protocols for RNA extraction and next-generation sequencing using miRNA and mRNA libraries. The protocol also includes RNA sequencing of Schwann cells, which allows the comparison between RNA content from cells and the secreted exosomes. Identification of RNAs present in Schwann cell-derived exosomes is a valuable tool to understand novel roles of Schwann cells in neuronal function in health and disease.
dc.languageen_US
dc.publisherHumana Press
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Chile
dc.sourceMethods in molecular biology (Clifton, N.J.), 1739, 299–315
dc.subjectExosomes
dc.subjectIntercellular communication
dc.subjectMicrovesicles
dc.subjectSchwann cell
dc.subjectTranscriptomics
dc.subjectmRNA
dc.titlePurification of Exosomes from Primary Schwann Cells, RNA Extraction, and Next-Generation Sequencing of Exosomal RNAs
dc.typeArtículos de revistas


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