A differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage

dc.creatorBeltran, Jorge F.
dc.creatorZamorano, Mauricio
dc.creatorHerrera Belen, Lisandra
dc.creatorRisopatron, Jennie
dc.creatorValdebenito, Ivan
dc.creatorNorambuena, Juan-Alejandro
dc.creatorFarias, Joaquin A.
dc.creatorFarias, Jorge G.
dc.date2021
dc.date2021-10-04T18:44:48Z
dc.date2021-10-04T18:44:48Z
dc.date.accessioned2022-10-18T14:52:27Z
dc.date.available2022-10-18T14:52:27Z
dc.identifierAQUACULTURE,Vol.545,,2021
dc.identifierhttp://repositoriodigital.uct.cl/handle/10925/4241
dc.identifier10.1016/j.aquaculture.2021.737211
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4444109
dc.descriptionSperm motility is a key event in fertilization, which is regulated by different factors. Among the factors that most affect sperm motility in fish are the external concentrations of Ca2+, the influence of which is highly regulated by various plasma membrane Ca2+-binding proteins. Concentrations of this cation have also been shown to be one of the most important factors affecting motility in cold-stored sperm cells. Taking into account the aforementioned aspects, we carried out a differential proteomic study on Salmo salar sperm stored at 4 degrees C with the aim of evaluating the protein profile on day 0 and day 14. The results of our study showed that 401 proteins were significantly downregulated (p < 0.05) on day 14, where four of them are key in the sperm motility of Salmo salar. The results of this study will allow a better understanding of the sperm activation mechanisms of Salmo salar, which will be of great importance in the design of future cold storage strategies for sperm preservation.
dc.languageen
dc.publisherELSEVIER
dc.sourceAQUACULTURE
dc.subjectSalmo salar
dc.subjectMotility
dc.subjectProteomics
dc.subjectChannel
dc.subjectCalcium
dc.titleA differential proteomic study reveals the downregulation of several plasma membrane Ca2+-binding proteins in the sperm of Atlantic salmon (Salmo salar) following cold storage
dc.typeArticle


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