Role of three plasma membrane Ca2+-binding proteins in the sperm motility of Atlantic salmon (Salmo salar)

dc.creatorBeltran, Jorge F.
dc.creatorFigueiras-Fierro, D.
dc.creatorZamorano, Mauricio
dc.creatorHerrera Belen, Lisandra
dc.creatorRisopatron, Jennie
dc.creatorValdebenito, Ivan
dc.creatorNorambuena, Juan-Alejandro
dc.creatorFarias, Jorge G.
dc.date2021
dc.date2021-10-04T18:54:33Z
dc.date2021-10-04T18:54:33Z
dc.date.accessioned2022-10-18T14:52:05Z
dc.date.available2022-10-18T14:52:05Z
dc.identifierAQUACULTURE,Vol.538,,2021
dc.identifierhttp://repositoriodigital.uct.cl/handle/10925/4358
dc.identifier10.1016/j.aquaculture.2021.736537
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4443967
dc.descriptionSalmonid sperm motility is regulated by different factors, such as ion concentration, osmotic pressure, pH, and temperature. The Ca2+ cation-dependent regulation and Ca2+-binding proteins are key regulatory mechanisms in the sperm activation process. In this study, the function of transient receptor potential ankyrin 1, transient receptor potential cation channel subfamily V member 4, and Na+/Ca2+ exchanger 1 on the sperm motility of Salmo salar were studied in vitro and in silico. Sperm inhibition assay results showed that the inhibition of the Na+/Ca2+ exchanger produced a significant decrease in total and progressive motility (p < 0.0001). The molecular docking-based analysis showed that inhibitor KB-R7943 binds strongly (-6.0 kcal/mol) in the Ca2+binding region in this exchanger. Additionally, it was demonstrated that, in contrast to Na+/Ca2+ exchange, neither transient receptor potential ankyrin 1 nor transient receptor potential cation channel subfamily V member 4 was directly associated with sperm motility. This research provides new and useful insights into the role of Ca2+-binding proteins present in the plasma membrane that regulate the sperm motility of Salmo salar.
dc.languageen
dc.publisherELSEVIER
dc.sourceAQUACULTURE
dc.subjectSalmo salar
dc.subjectMotility
dc.subjectIn vitro
dc.subjectIn silico
dc.subjectChannel
dc.subject
dc.titleRole of three plasma membrane Ca2+-binding proteins in the sperm motility of Atlantic salmon (Salmo salar)
dc.typeArticle


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