Formation of biofilms of the salmon pathogen Flavobacterium psychrophilum in differents surfaces using the CDC biofilm reactor

Formación de biopelículas del salmón patógeno Flavobacterium psychrophilum en diferentes superficies utilizando el reactor biofilm CDC

dc.creatorVidal, José M.
dc.creatorMiranda, Claudio D.
dc.creatorFuente, Mery De la
dc.creatorAlarcón, Manuel
dc.creatorAroca, Germán
dc.creatorSossa, Katherine
dc.creatorRuiz, Pamela
dc.creatorUrrutia, Homero
dc.date2020-05-25T22:56:10Z
dc.date2020-05-25T22:56:10Z
dc.date2020-01-01
dc.date.accessioned2022-10-18T12:06:52Z
dc.date.available2022-10-18T12:06:52Z
dc.identifierAquaculture, Volume 514, 1 January 2020, 734459
dc.identifier0044-8486
dc.identifierhttp://repositoriodigital.ucsc.cl/handle/25022009/1632
dc.identifier10.1016/j.aquaculture.2019.734459
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/4441270
dc.descriptionArtículo de publicación ISI
dc.descriptionAquaculture settings present a number of surfaces that can potentially act as a reservoir for Flavobacterium psychrophilum, resulting in a serious threat to the health of farmed salmonids. The aim of this study was to employ the CDC Biofilm Reactor to evaluate the capacity of F. psychrophilum to form biofilms on stainless steel, polystyrene, polyurethane, and polycarbonate. Scanning electron microscopy and confocal laser scanning microscopy were used to assess biofilm formation, while epifluorescence and qPCR methods were effectively used to determine cell contents. After a 72 h incubation period, F. psychrophilum had formed mature biofilms composed by dense, multilayered clusters of short bacilli on stainless steel, polystyrene, and polycarbonate. Nevertheless, biofilms formed on stainless steel presented a significantly higher cell density than those formed on polyurethane. Biofilm growth was poor on polyurethane surfaces. These findings will facilitate the design of appropriate guidelines for more efficiently managing F. psychrophilum and controlling the persistence of this pathogen in fish-farming environments.
dc.languageen
dc.publisherElsevier
dc.sourcehttps://doi.org/10.1016/j.aquaculture.2019.734459
dc.subjectBiofilms
dc.subjectBiofilm reactor
dc.subjectCDC
dc.subjectFish pathogen
dc.subjectFlavobacterium psychrophilum
dc.subjectStainless steel
dc.titleFormation of biofilms of the salmon pathogen Flavobacterium psychrophilum in differents surfaces using the CDC biofilm reactor
dc.titleFormación de biopelículas del salmón patógeno Flavobacterium psychrophilum en diferentes superficies utilizando el reactor biofilm CDC
dc.typeArtículos de revistas


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