dc.creator | Calvo, Rafael | |
dc.creator | Espinosa, Maximiliano | |
dc.creator | Figueroa, David | |
dc.creator | Pozo, Luz María | |
dc.creator | Conget, Paulette | |
dc.date.accessioned | 2019-07-09T13:31:11Z | |
dc.date.accessioned | 2022-10-17T17:51:49Z | |
dc.date.available | 2019-07-09T13:31:11Z | |
dc.date.available | 2022-10-17T17:51:49Z | |
dc.date.created | 2019-07-09T13:31:11Z | |
dc.date.issued | 2018 | |
dc.identifier | Calvo, R., Espinosa, M., Figueroa, D., Pozo, L. M., & Conget, P. (2018). Assessment of Cell Viability of Fresh Osteochondral Allografts in N-Acetylcysteine-Enriched Medium. CARTILAGE. https://doi.org/10.1177/1947603518786547 | |
dc.identifier | doi.org/10.1177/1947603518786547 | |
dc.identifier | http://hdl.handle.net/11447/2510 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/4422694 | |
dc.description.abstract | Objective The purpose of this study was to evaluate the effect of N-acetylcysteine (NAC)-enriched storage medium on fresh osteochondral viability at 4°C. Our hypothesis was that the cell viability of chondrocytes obtained from human osteochondral tissue and stored at 4°C significantly improves in the presence of NAC. Design Controlled laboratory study. For this study, 8 samples of femoral condyle osteochondral tissue were obtained from patients undergoing total knee replacement. The samples were stored at either 4°C in phosphate-buffered saline (PBS) or at 3 different concentrations of NAC (NAC 1, 2, and 5 mM). Cell viability was analyzed at time 0 and 4 weeks by flow cytometry. The results of cell viability (median) were analyzed statistically using analysis of variance and Tukey's post hoc test. P values <0.05 were considered statistically significant. Results The viability at time 0 was 95.5% ± 3.7%. At 4 weeks, the cell viability was 56.8% ± 20.1% in the control group (PBS), 83.8% ± 11.9% in the group stored with NAC 1 mM, 73.4% ± 13.6% in the group stored with NAC 2 mM, and 66.4% ± 27.7% in the group stored with NAC 5 mM. A statistically significant difference from the baseline viability (time 0) was observed in the PBS control group ( P = 0.0018) but not in the other groups. A statistically significant difference was observed in the NAC 1 mM group compared with the PBS group ( P = 0.0255). Conclusion The use of NAC at 1 mM concentration improves cell viability after 4 weeks of storage in chondrocytes obtained from human osteochondral tissue. | |
dc.language | en | |
dc.publisher | Sage | |
dc.subject | Chondrocyte viability | |
dc.subject | Osteochondral allografts | |
dc.subject | Storage medium | |
dc.subject | Tissue banking | |
dc.title | Assessment of Cell Viability of Fresh Osteochondral Allografts in N-Acetylcysteine-Enriched Medium | |
dc.type | Article | |