info:eu-repo/semantics/article
Lactobacillus casei beneficially modulates immuno-coagulative response in an endotoxemia model
Fecha
2018-01Registro en:
Haro, Ana Cecilia; Monaco, Maria Eugenia; Medina, Marcela Susana; Lactobacillus casei beneficially modulates immuno-coagulative response in an endotoxemia model; Lippincott Williams; Blood Coagulation & Fibrinolysis : An International Journal In Haemostasis And Thrombosis.; 29; 1; 1-2018; 104-110
0957-5235
1473-5733
CONICET Digital
CONICET
Autor
Haro, Ana Cecilia
Monaco, Maria Eugenia
Medina, Marcela Susana
Resumen
The current study aims at evaluating the effect of the oral administration of Lactobacillus casei CERELA (CRL) 431 on parameters implicated in inflammation-coagulation interaction using a model of acute inflammation induced by lipopolysaccharide (LPS) in mice. Six-week-old Balb/c mice were treated with L. casei for 5 consecutive days. Then treated and untreated mice received an LPS injection (L. caseiRLPS and LPS groups, respectively). Liver and kidney were removed, blood samples were obtained, and hemostatic and inflammatory parameters were evaluated at different times post LPS injection. Preventive L. casei administration induced a significant decrease in proinflammatory TNF-A and IL-6 cytokines by decreasing tissue factor expression in liver and kidney. Moreover, the lower expression of tissue factor in the L. caseiRLPS group led to a lower activation of the coagulation system, which was observed by the fast systemic restoration of factors VII and V coagulation factors and antithrombin levels. This study highlights the capacity of L. casei to modulate the hemostatic unbalance in an acute endotoxemia model. Our findings showed the ability of L. casei CRL 431 to regulate the immuno-coagulative response. This fact could be helpful to propose new adjunctive strategies addressed to the restoration of physiological anticoagulant mechanisms in sepsis patients.