info:eu-repo/semantics/article
Trypanosoma cruzi: Multiple nucleoside diphosphate kinase isoforms in a single cell
Fecha
2008-09Registro en:
Miranda, Mariana Reneé; Canepa, Gaspar Exequiel; Bouvier, León Alberto; Pereira, Claudio Alejandro; Trypanosoma cruzi: Multiple nucleoside diphosphate kinase isoforms in a single cell; Academic Press Inc Elsevier Science; Experimental Parasitology; 120; 1; 9-2008; 103-107
0014-4894
CONICET Digital
CONICET
Autor
Miranda, Mariana Reneé
Canepa, Gaspar Exequiel
Bouvier, León Alberto
Pereira, Claudio Alejandro
Resumen
Nucleoside diphosphate kinases (NDPKs) are multifunctional enzymes involved mainly in the conservation of nucleotides and deoxynucleotides at intracellular levels. Here we report the characterization of two NDPKs from the protozoan parasite Trypanosoma cruzi, the etiological agent of Chagas disease. TcNDPK1 and TcNDPK2 were biochemically characterized presenting different kinetic parameters and regulation mechanisms. NDPK activity was mainly detected in soluble fractions according to the digitonin extraction technique; however 20% of the activity remains insoluble at digitonin concentrations up to 5mgml(-1). TcNDPK1 is a short enzyme isoform, whereas TcNDPK2 is a long one containing a DM10 motif. In addition, two other putative NDPK genes (TcNPDK3 and TcNDPK4) were detected by data mining at the T. cruzi genome database. The large number and diversity of NDPK isoforms are in agreement with those previously observed for other T. cruzi phosphotransferases, such as adenylate kinases.