info:eu-repo/semantics/article
Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina
Date
2016-10-18Registration in:
Aguilera Sammaritano, Juan Alberto; Lopez Lastra, Claudia Cristina; Leclerque, Andreas; Vazquez, Fabio; Toro, Maria Eugenia; et al.; Control of Bemisia tabaci by entomopathogenic fungi isolated from arid soils in Argentina; Taylor & Francis Ltd; Biocontrol Science and Technology; 26; 12; 18-10-2016; 1668-1682
0958-3157
1360-0478
CONICET Digital
CONICET
Author
Aguilera Sammaritano, Juan Alberto
Lopez Lastra, Claudia Cristina
Leclerque, Andreas
Vazquez, Fabio
Toro, Maria Eugenia
D´Alessandro, Celeste Paola
Cuthbertson, Andrew G.S.
Lechner, Bernardo Ernesto
Abstract
Entomopathogenic Hypocreales were isolated from arid soils in Argentina using Tenebrio molitor as bait and tested for their biological performance at 30°C and 45–65% RH. Conidial germination was tested in three vegetable oils (sunflower, olive and maize) at two concentrations (1% and 10%) to evaluate their compatibility for further liquid formulations. According to radial growth and germination results, we selected four isolates to test their pathogenicity against second instar B. tabaci nymphs with the selected oil formulations at 30°C. CEP381 and CEP401 showed the highest radial growth. Isolates CEP381, CEP401, CEP413 and CEP409 (Metarhizium spp.) had similar germination percentages as compared with water control when germinated on either sunflower, olive or maize oils at 10% v/v. The highest mortality of B. tabaci was observed for the isolates CEP381 in sunflower oil and CEP401 in olive oil. Molecular identification of isolates was performed using ITS4–5 primers. All isolates belong to the Metarhizium core group. Tested isolates could grow and infect B. tabaci nymphs at 30°C in some of the vegetable oils as carriers, providing new possibilities for integrated pest management of Bemisia tabaci.