info:eu-repo/semantics/article
Interplay between neutrophils and trophoblast cells conditions trophoblast function and triggers vascular transformation signals
Fecha
2019-09-26Registro en:
Calo, Guillermina; Sabbione, Florencia; Pascuali, Natalia Marisa; Keitelman, Irene Angélica; Vota, Daiana Marina; et al.; Interplay between neutrophils and trophoblast cells conditions trophoblast function and triggers vascular transformation signals; Wiley-liss, Div John Wiley & Sons Inc; Journal of Cellular Physiology; 22; 6; 26-9-2019
0021-9541
CONICET Digital
CONICET
Autor
Calo, Guillermina
Sabbione, Florencia
Pascuali, Natalia Marisa
Keitelman, Irene Angélica
Vota, Daiana Marina
Paparini, Daniel Esteban
Ramhorst, Rosanna Elizabeth
Parborell, Maria Fernanda Agustina
Trevani, Analía
Pérez Leirós, Claudia
Resumen
Normal placentation entails highly regulated interactions of maternal leukocytes with vascular and trophoblast cells to favor vascular transformation. Neutrophil activation and neutrophil extracellular trap (NET) formation associate with poor placentation and severe pregnancy complications. To deepen into the mechanisms of trophoblast-neutrophil interaction, we explored the effects of NETs on trophoblast cell function and, conversely, whether trophoblast cell-derived factors condition neutrophils to favor angiogenesis and anti-inflammatory signals required for fetal growth. NETs isolated from activated neutrophils hindered trophoblast cell migration. Trophoblast conditioned media prevented the effect as well as the vasoactive intestinal peptide (VIP) known to regulate trophoblast and neutrophil function. On the other hand, factors released by trophoblast cells and VIP shaped neutrophils to a proangiogenic profile with increased vascular endothelial growth factor synthesis and increased capacity to promote vascular transformation. Results presented here provide novel clues to reconstruct the interaction of trophoblast cells and neutrophils in vivo during placentation in humans.