info:eu-repo/semantics/article
Amorphous calcium organophosphate nanoshells as potential carriers for drug delivery to Ca2+-enriched surfaces
Fecha
2020-04-08Registro en:
Perez Enriquez, Darlin Johana; Dell'arciprete, Maria Laura; Dittler, Maria Laura; Miñan, Alejandro Guillermo; Prieto, Eduardo Daniel; et al.; Amorphous calcium organophosphate nanoshells as potential carriers for drug delivery to Ca2+-enriched surfaces; Royal Society of Chemistry; New Journal of Chemistry; 44; 18; 08-4-2020; 7541-7551
1144-0546
1369-9261
CONICET Digital
CONICET
Autor
Perez Enriquez, Darlin Johana
Dell'arciprete, Maria Laura
Dittler, Maria Laura
Miñan, Alejandro Guillermo
Prieto, Eduardo Daniel
Gonzalez, Monica Cristina
Resumen
A highly selective nanocarrier for targeted drug transport and delivery to calcium-containing surfaces, as a bone mineral matrix, is described. The nanocarrier, a calcium phosphate (CaP) nanoshell, is capable of interacting with calcium ions contained in enriched surfaces (Ca2+ modified mica surface, hydroxyapatite nanoparticle (Ap) films on glass, and Ap modified 45S5® bioactive glass-based scaffolds) with the consequent disruption of the inorganic structure and release of (bio) molecules contained in the interior. The antibiotic Levofloxacin (LX) was used as a model drug for encapsulation and drug release studies which allowed monitoring by fluorescence spectroscopic methods. The accumulation and disruption of CaP nanoshells triggered by calcium ions over surfaces were followed by microscopy techniques such as SEM, AFM, and fluorescence microscopy. Bacterial susceptibility and time killing assays demonstrated the bactericidal potential of the nanoshells containing LX. A mechanism for the Ca2+-activated CaP nanoshell accumulation and drug release is proposed and discussed.